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Sample GSM416131 Query DataSets for GSM416131
Status Public on Jul 28, 2009
Title non-tumor pleura_6012
Sample type genomic
 
Source name non-malignant pulmonary tissue
Organism Homo sapiens
Characteristics disease state: control
age: 73
gender: male
Extracted molecule genomic DNA
Extraction protocol DNA from fresh frozen tissue was isolated with QIAamp DNA mini kit (Qiagen, Valencia, CA)
Label Cy3,Cy5
Label protocol DNA was sodium bisulfite modified using the EZ DNA Methylation Kit (Zymo Research, Orange, CA).
 
Hybridization protocol Illumina GoldenGate® methylation bead arrays interrogated 1505 CpG loci associated with 803 cancer-related genes processed at the UCSF Institute for Human Genetics, Genomics Core Facility as described in Bibikova et al. High-throughput DNA methylation profiling using universal bead arrays. Genome research 2006;16:383-93.
Bisulfite-converted DNAs are biotinylated and hybridized to query oligos, and washed. The hybridized oligos are then extended and ligated to create amplifiable templates with methylation-specific primers. The PCR that follows uses fluorescently labeled universal PCR primers.
Scan protocol Arrays were imaged using a BeadArray Reader scanner (Bibikova et al. High-throughput DNA methylation profiling using universal bead arrays. Genome research 2006;16:383-93.)
Description no additional information
Data processing Illumina BeadStudio Methylation software (version 3.2.0) was used for dataset assembly. Fluorescent signals for methylated (Cy5) and unmethylated (Cy3) alleles give methylation level (β) = (max(Cy5 , 0))/(|Cy3| + |Cy5| + 100) with ~30 replicate bead measurements per locus. The β-value provides a continuous measure of levels of DNA methylation in samples, ranging from 0 in the case of completely unmethylated sites to 1 in completely methylated sites. Detection P-values determined poor performing samples (n=2) and CpG loci (n=8), which were removed from analysis. X chromosome loci were also removed, leaving 1413 CpG loci associated with 773 genes. Raw average beta values were used and not subjected to normalization as per manufacturer's recommendations.
 
Submission date Jun 11, 2009
Last update date Apr 13, 2010
Contact name Brock Clarke Christensen
E-mail(s) brock.clarke.christensen@dartmouth.edu
Organization name Dartmouth Medical School
Street address 7650 Remsen
City Hanover
State/province NH
ZIP/Postal code 03755
Country USA
 
Platform ID GPL9183
Series (1)
GSE16559 Lung adenocarcinoma and mesothelioma DNA methylation

Data table header descriptions
ID_REF
VALUE raw average beta values (continuous and bounded by 0 & 1)

Data table
ID_REF VALUE
AATK_E63_R 0.96474
AATK_P519_R 0.88493
AATK_P709_R 0.77835
ABCA1_E120_R 0.02669
ABCA1_P45_F 0.04505
ABCB4_E429_F 0.96369
ABCB4_P51_F 0.965
ABCB4_P892_F 0.87735
ABCC2_E16_R 0.85641
ABCC2_P88_F 0.96471
ABCC5_P444_F 0.03731
ABCG2_P178_R 0.14706
ABCG2_P310_R 0.02347
ABL1_P53_F 0.12818
ABL2_P459_R 0.35229
ABO_E110_F 0.19163
ABO_P312_F 0.11308
ACTG2_E98_R 0.8702
ACTG2_P346_F 0.73544
ACTG2_P455_R 0.95389

Total number of rows: 1413

Table truncated, full table size 28 Kbytes.




Supplementary data files not provided
Processed data not applicable for this record

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