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Sample GSM418747 Query DataSets for GSM418747
Status Public on Jan 31, 2011
Title 5290_CNTL
Sample type RNA
 
Source name 5290, control, skin fibroblasts
Organism Homo sapiens
Characteristics cell line: 5290, control, skin fibroblasts
gender: female
age at biopsy: 7 years
Extracted molecule total RNA
Extraction protocol Total RNA was prepared using TRIzol (Invitrogen) and purified on Qiagen RNeasy Mini columns (Qiagen), according to manufacturers' instructions.
Label biotin
Label protocol Target preparations were performed as described by the Affymetrix GeneChip Expression Analysis Technical Manual (http://www.affymetrix.com), and were conducted at the DNA array facility of the University of Lausanne (Switzerland). 3 ug of each pool was used to perform the chiparray experiment according to the Affymetrix Gene Expression procedure.
 
Hybridization protocol Twelve micrograms of biotinylated cRNA from each sample were fragmented and hybridized to GeneChip Mouse 430 2.0 arrays, according to standard procedures. Washing and staining was performed using the EuGE-WS2v5 protocol.
Scan protocol Scanning was done on an Affymetrix GeneChip Scanner 7G and hybridization quality control were assessed using RACE (Psarros et al., 2005), a web service based on Bioconductor “affy” and “affyPLM” packages (Gautier et al., 2004).
Description 5290_CNTL
Data processing Normalized expression signals were calculated from Affymetrix CEL files using RMA (Irizarry et al., 2003).
 
Submission date Jun 19, 2009
Last update date Sep 01, 2016
Contact name Charlotte N. Henrichsen
Organization name University of Lausanne
Department Center for Integrative Genomics
Street address Quartier Sorge
City Lausanne
ZIP/Postal code CH-1015
Country Switzerland
 
Platform ID GPL570
Series (1)
GSE16715 Expression profiling in Williams-Beuren Syndrome patient fibroblast cell lines
Relations
Reanalyzed by GSE86362

Data table header descriptions
ID_REF
VALUE Log2 normalized expression signal

Data table
ID_REF VALUE
1007_s_at 6.512225168
1053_at 4.108123606
117_at 2.569299678
121_at 5.906633388
1255_g_at 1.987638743
1294_at 4.494004173
1316_at 3.742536691
1320_at 4.111638909
1405_i_at 2.134249537
1431_at 2.271725766
1438_at 4.534627904
1487_at 4.89454155
1494_f_at 3.836222205
1552256_a_at 4.541066783
1552257_a_at 6.373623005
1552258_at 2.779208094
1552261_at 3.025399595
1552263_at 3.393049802
1552264_a_at 6.225289166
1552266_at 2.120542366

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM418747.CEL.gz 7.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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