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| Status |
Public on Jan 31, 2011 |
| Title |
Expression profiling in Williams-Beuren Syndrome patient fibroblast cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Williams-Beuren Syndrome (WBS) is a neurodevelopmental disorder caused by aa 1.5 Mb microdeletion on human chromosome 7. Although the molecular cause of the disorder is well-established, little is known about the global impact of the deletion on gene expression. Here we profiled the transcriptomes of fibroblast cell lines from 8 young girls with WBS, and 9 sex- and age-matched control individuals
Keywords: disease state analysis, gene expression profiling
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| Overall design |
Skin fibroblast of 8 WBS and 9 control individuals were obtained from the cell culture collections of the “Centre de Biotechnologie Cellulaire, Hospices Civils de Lyon, Hôpital Debrousse”, Lyon, France. Appropriate informed consent was obtained for each sample by the physicians in charge. Human skin fibroblasts were grown in HAM F-10, supplemented with 10% fetal bovine serum and 1% antibiotics (Invitrogen). Total RNA was prepared using TriZOL Reagent (Invitrogen) and RNeasy Mini Columns (Qiagen) according to the manufacturers’ instructions.
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| Contributor(s) |
Henrichsen CN, Csardi G, Zabot M, Bergmann S, Merla G, Reymond A |
| Citation(s) |
21304579 |
| |
| Submission date |
Jun 19, 2009 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Charlotte N. Henrichsen |
| Organization name |
University of Lausanne
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| Department |
Center for Integrative Genomics
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| Street address |
Quartier Sorge
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| City |
Lausanne |
| ZIP/Postal code |
CH-1015 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA117473 |
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