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Sample GSM418750 Query DataSets for GSM418750
Status Public on Jan 31, 2011
Title 8495_CNTL
Sample type RNA
 
Source name 8495, control, skin fibroblasts
Organism Homo sapiens
Characteristics cell line: 8495, control, skin fibroblasts
gender: female
age at biopsy: 5 years
Extracted molecule total RNA
Extraction protocol Total RNA was prepared using TRIzol (Invitrogen) and purified on Qiagen RNeasy Mini columns (Qiagen), according to manufacturers' instructions.
Label biotin
Label protocol Target preparations were performed as described by the Affymetrix GeneChip Expression Analysis Technical Manual (http://www.affymetrix.com), and were conducted at the DNA array facility of the University of Lausanne (Switzerland). 3 ug of each pool was used to perform the chiparray experiment according to the Affymetrix Gene Expression procedure.
 
Hybridization protocol Twelve micrograms of biotinylated cRNA from each sample were fragmented and hybridized to GeneChip Mouse 430 2.0 arrays, according to standard procedures. Washing and staining was performed using the EuGE-WS2v5 protocol.
Scan protocol Scanning was done on an Affymetrix GeneChip Scanner 7G and hybridization quality control were assessed using RACE (Psarros et al., 2005), a web service based on Bioconductor “affy” and “affyPLM” packages (Gautier et al., 2004).
Description 8495_CNTL
Data processing Normalized expression signals were calculated from Affymetrix CEL files using RMA (Irizarry et al., 2003).
 
Submission date Jun 19, 2009
Last update date Sep 01, 2016
Contact name Charlotte N. Henrichsen
Organization name University of Lausanne
Department Center for Integrative Genomics
Street address Quartier Sorge
City Lausanne
ZIP/Postal code CH-1015
Country Switzerland
 
Platform ID GPL570
Series (1)
GSE16715 Expression profiling in Williams-Beuren Syndrome patient fibroblast cell lines
Relations
Reanalyzed by GSE86362

Data table header descriptions
ID_REF
VALUE Log2 normalized expression signal

Data table
ID_REF VALUE
1007_s_at 6.401236182
1053_at 3.43924274
117_at 2.534305928
121_at 5.683767209
1255_g_at 2.022988107
1294_at 5.641774145
1316_at 3.567264364
1320_at 3.876823824
1405_i_at 2.021798517
1431_at 2.20380469
1438_at 5.203258029
1487_at 4.926474476
1494_f_at 3.836129218
1552256_a_at 4.745073705
1552257_a_at 5.933710961
1552258_at 2.534058391
1552261_at 2.699646201
1552263_at 3.531694312
1552264_a_at 5.945340998
1552266_at 2.532304115

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM418750.CEL.gz 7.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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