|
Status |
Public on Jan 31, 2011 |
Title |
10590_WBS |
Sample type |
RNA |
|
|
Source name |
10590, WBS, skin fibroblasts
|
Organism |
Homo sapiens |
Characteristics |
cell line: 10590, WBS, skin fibroblasts microdeletion: del(7q11.23) gender: female age at biopsy: 3 years
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was prepared using TRIzol (Invitrogen) and purified on Qiagen RNeasy Mini columns (Qiagen), according to manufacturers' instructions.
|
Label |
biotin
|
Label protocol |
Target preparations were performed as described by the Affymetrix GeneChip Expression Analysis Technical Manual (http://www.affymetrix.com), and were conducted at the DNA array facility of the University of Lausanne (Switzerland). 3 ug of each pool was used to perform the chiparray experiment according to the Affymetrix Gene Expression procedure.
|
|
|
Hybridization protocol |
Twelve micrograms of biotinylated cRNA from each sample were fragmented and hybridized to GeneChip Mouse 430 2.0 arrays, according to standard procedures. Washing and staining was performed using the EuGE-WS2v5 protocol.
|
Scan protocol |
Scanning was done on an Affymetrix GeneChip Scanner 7G and hybridization quality control were assessed using RACE (Psarros et al., 2005), a web service based on Bioconductor “affy” and “affyPLM” packages (Gautier et al., 2004).
|
Description |
10590_WBS
|
Data processing |
Normalized expression signals were calculated from Affymetrix CEL files using RMA (Irizarry et al., 2003).
|
|
|
Submission date |
Jun 19, 2009 |
Last update date |
Sep 01, 2016 |
Contact name |
Charlotte N. Henrichsen |
Organization name |
University of Lausanne
|
Department |
Center for Integrative Genomics
|
Street address |
Quartier Sorge
|
City |
Lausanne |
ZIP/Postal code |
CH-1015 |
Country |
Switzerland |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE16715 |
Expression profiling in Williams-Beuren Syndrome patient fibroblast cell lines |
|
Relations |
Reanalyzed by |
GSE86362 |