|
| Status |
Public on Jan 31, 2011 |
| Title |
10590_WBS |
| Sample type |
RNA |
| |
|
| Source name |
10590, WBS, skin fibroblasts
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: 10590, WBS, skin fibroblasts
microdeletion: del(7q11.23)
gender: female
age at biopsy: 3 years
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using TRIzol (Invitrogen) and purified on Qiagen RNeasy Mini columns (Qiagen), according to manufacturers' instructions.
|
| Label |
biotin
|
| Label protocol |
Target preparations were performed as described by the Affymetrix GeneChip Expression Analysis Technical Manual (http://www.affymetrix.com), and were conducted at the DNA array facility of the University of Lausanne (Switzerland). 3 ug of each pool was used to perform the chiparray experiment according to the Affymetrix Gene Expression procedure.
|
| |
|
| Hybridization protocol |
Twelve micrograms of biotinylated cRNA from each sample were fragmented and hybridized to GeneChip Mouse 430 2.0 arrays, according to standard procedures. Washing and staining was performed using the EuGE-WS2v5 protocol.
|
| Scan protocol |
Scanning was done on an Affymetrix GeneChip Scanner 7G and hybridization quality control were assessed using RACE (Psarros et al., 2005), a web service based on Bioconductor “affy” and “affyPLM” packages (Gautier et al., 2004).
|
| Description |
10590_WBS
|
| Data processing |
Normalized expression signals were calculated from Affymetrix CEL files using RMA (Irizarry et al., 2003).
|
| |
|
| Submission date |
Jun 19, 2009 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Charlotte N. Henrichsen |
| Organization name |
University of Lausanne
|
| Department |
Center for Integrative Genomics
|
| Street address |
Quartier Sorge
|
| City |
Lausanne |
| ZIP/Postal code |
CH-1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE16715 |
Expression profiling in Williams-Beuren Syndrome patient fibroblast cell lines |
|
| Relations |
| Reanalyzed by |
GSE86362 |
