|
| Status |
Public on Feb 27, 2020 |
| Title |
MG cells, replication 2 |
| Sample type |
RNA |
| |
|
| Source name |
colon cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCT116 cells
group: high migratory cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was extracted using miRNeasy kit (Qiagen) according to the manufacturer’s protocol.
|
| Label |
Cy3
|
| Label protocol |
Sample labeling, array processing and scanning, and data extraction were performed as described in One-Color Microarray-Based Gene Expression Analysis’ Protocol. These protocols are available for download from Agilent's DNA Microarray Manuals website. For microarray analysis, total RNA (400 ng) was used for amplification and labeling of complementary RNA (cRNA) using a Low RNA Input Liner Amplification Kit PLUS and a succinimide-containing fluorescent dye (Cy3)-CTP according to the manufacturer’s protocol (Agilent).
|
| |
|
| Hybridization protocol |
Hybridizations were performed using GE Hybridization Kit (P/N 5188-5242) and GE Wash Buffer 1 and 2 (P/N 5188-5325, -5326) as following Agilent's protocol. The fragmentation mixture and hybridized to Agilent Whole Human Genome Oligo Microarrays (G4858A) for 17 hours at 65C in a rotating Agilent hybridization oven.
|
| Scan protocol |
Microarrays were scanned using Agilent’s DNA Microarray Scanner (P/N G2600D).
|
| Description |
control
|
| Data processing |
Data extracted using Agilent's Feature Extraction software. Raw data output was imported into Genespring GX14.9 (Agilent) and normalized each chip to the 75th percentile of all measurements (per chip normalization).
|
| |
|
| Submission date |
Dec 02, 2019 |
| Last update date |
Feb 27, 2020 |
| Contact name |
Kensei Nishida |
| E-mail(s) |
knishida@tokushima-u.ac.jp
|
| Organization name |
Tokushima University Graduate school
|
| Street address |
3-18-15 Kuramoto-cho
|
| City |
Tokushima |
| State/province |
Tokushima |
| ZIP/Postal code |
7708503 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18509 |
| Series (1) |
| GSE141240 |
miRNA expression in high migratory cells |
|
