|
| Status |
Public on Feb 01, 2010 |
| Title |
251186836388 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
191-cy3
|
| Organism |
Rattus norvegicus |
| Characteristics |
label: 191-cy3
extract: 191
sample: T191
subject: CMO 191
tissue: thoracic aorta
origin: control mother
extract_amount: 5.1
extract_concentration: 176.0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy3
|
| Label protocol |
cRNA Agilent
|
| |
|
| Channel 2 |
| Source name |
197-cy5
|
| Organism |
Rattus norvegicus |
| Characteristics |
label: 197-cy5
extract: 197
sample: 197
subject: DMO 197
tissue: thoracic aorta
origin: diabetic mother
extract_amount: 5.3
extract_concentration: 50.0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy5
|
| Label protocol |
cRNA Agilent
|
| |
|
| |
| Hybridization protocol |
Agilent : 750.0 ng at 60 degree_C during 16 hours
|
| Scan protocol |
Scanned on an GenePix 4000B fluorescent scanner. Image intensity data were extracted with GenePix Pro 6.0 analysis software.
|
| Description |
191-cy3 vs 197-cy5
Date:2005/10/03
|
| Data processing |
Interslide norm. : quantile, Intraslide norm. : loess, Background norm. : Kooperberg, Software used : limmagui --> VALUE data obtained from log of processed Red signal/processed Green signal.
|
| |
|
| Submission date |
Jun 23, 2009 |
| Last update date |
Nov 26, 2009 |
| Contact name |
Celine Fassot-Lucht |
| E-mail(s) |
celine.fassot@inserm.fr
|
| Organization name |
INSERM U1083
|
| Department |
BNMI
|
| Street address |
Rue Haute de Reculée
|
| City |
Angers |
| ZIP/Postal code |
49045 cedex 01 |
| Country |
France |
| |
|
| Platform ID |
GPL890 |
| Series (1) |
| GSE16750 |
Aortic gene expression profile of rats exposed in utero to maternal diabetes |
|
