|
| Status |
Public on Dec 07, 2020 |
| Title |
PUFA 14 High diet |
| Sample type |
SRA |
| |
|
| Source name |
uterine endometrial tissue
|
| Organism |
Bos taurus |
| Characteristics |
treatment: pufa
diet: High diet
|
| Treatment protocol |
Heifers were offered a concentrate-based diet supplemented (n-3 PUFA; n=32) or not (Control; n=28) with an n-3 PUFA-enriched product for 30 days prior to insemination. Following insemination, animals were allocated, within treatment, to one of two post insemination diets, either remaining on their existing high plane of nutrition (Control + High (n = 14); n-3 PUFA + High (n = 15)), or fed a low plane of nutrition diet (Control + Low (n = 14); n-3 PUFA + Low (n = 17)).
|
| Growth protocol |
Estrous cycles in normal nulliparous crossbred beef heifers (n=60) were synchronized in reproductively. Heifers were offered a concentrate-based diet supplemented (n-3 PUFA; n=32) or not (Control; n=28) with an n-3 PUFA-enriched product for 30 days prior to insemination. Following insemination, animals were allocated, within treatment, to one of two post insemination diets, either remaining on their existing high plane of nutrition (Control + High (n = 14); n-3 PUFA + High (n = 15)), or fed a low plane of nutrition diet (Control + Low (n = 14); n-3 PUFA + Low (n = 17)).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from frozen uterine endometrial tissue by homogenisation using the Qiagen Rneasy RNA isolation kit.
Single-end mRNA-seq libraries were constructed using the Illumina Truseq sample prep kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
PUFA-supplemented, high diet post-insemination
JE5-8320
|
| Data processing |
Basecalls performed using CASAVA version 1.4
Input reads were then aligned to the bovine reference genome (UMD3.1) using TopHat (v2.0.9).
HTSeq (v0.5.4p5) was employed to calculate the number of sequence reads overlapping all protein-coding genes from the ENSEMBL v74 annotation of the bovine genome.
Genome_build: UMD 3.1
Supplementary_files_format_and_content: Tab delimited txt file of merged gene read counts
|
| |
|
| Submission date |
Dec 12, 2019 |
| Last update date |
Dec 07, 2020 |
| Contact name |
Kate Keogh |
| Organization name |
Teagasc Grange
|
| Department |
Animal and Bioscience Department
|
| Street address |
Dunsany
|
| City |
Co. Meath |
| ZIP/Postal code |
0000 |
| Country |
Ireland |
| |
|
| Platform ID |
GPL15749 |
| Series (1) |
| GSE141937 |
Effect of dietary n-3 PUFA supplementation and post-insemination plane of nutrition on global gene expression patterns in bovine uterine endometrial tissue |
|
| Relations |
| BioSample |
SAMN13549425 |
| SRA |
SRX7354727 |
