|
| Status |
Public on Dec 17, 2019 |
| Title |
Vc-10nMRA-2 |
| Sample type |
SRA |
| |
|
| Source name |
Mouse embryonic fibroblast
|
| Organism |
Mus musculus |
| Characteristics |
strain: OG2
cell type: Mouse embryonic fibroblast
passage: 2
treatment: Vc-, 10nMRA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using bowtie
Transcripts Per Million were calculated using a protocol from RSEM
Genome_build: mm10
Supplementary_files_format_and_content: TPM
|
| |
|
| Submission date |
Dec 16, 2019 |
| Last update date |
Dec 17, 2019 |
| Contact name |
Zheng Hui |
| E-mail(s) |
zhang_mengdan@gibh.ac.cn
|
| Organization name |
GIBH
|
| Street address |
Kaiyuan Avenue 190
|
| City |
Guangzhou |
| ZIP/Postal code |
510530 |
| Country |
China |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE142121 |
Interaction of Va and Vc in somatic cell reprogramming |
|
| Relations |
| BioSample |
SAMN13575952 |
| SRA |
SRX7388429 |
