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Status |
Public on Jun 21, 2020 |
Title |
brip1brip2 BRM-GFP-IP-bio1 |
Sample type |
SRA |
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Source name |
Young seedlings
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Organism |
Arabidopsis thaliana |
Characteristics |
genotype: brip1brip2 tissue: Seedling developmental stage: 14-day-old antibody: GFP (ab290, abcam)
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Growth protocol |
Plants were grown in 1/2MS media with 22 degree in 16h light/8h dark
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Extracted molecule |
genomic DNA |
Extraction protocol |
Lysates were clarified from sonicated protein-DNA complexes were isolated with GFP antibody. Two ng of ChIP DNA immuoprecipitated by anti-GFP antibody was used for ChIP-seq library construction. Blunting end, adapter ligation and amplification were carried out using Illumina Genomic DNA Sample Prep Kits according to the manufacturer's protocol. Illumina Novaseq was used for high-throughput sequencing of the ChIP-seq library.
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Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina NovaSeq 6000 |
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Data processing |
The raw reads were mapped to the reference genome (Arabidopsis thaliana genome, TAIR10) with Bowtie for Illumina (Galaxy Version 1.1.2) for DNA sequencing on web https://usegalaxy.org/ with default setting, except that the paired-end was required. MACS2 (Galaxy Version 2.1.1) was used for peak calling using the following parameters “gsize=119,667,750, bw=300, q=0.01, nomodel, extsize=200”. The alignments were first converted to Wiggle (WIG) files and the bigwig files were generated using bamCoverage with “-bs 10”, “--normalizeUsing RPKM (reads per kilobase per million)” in DeepTools and then the data were imported into the Integrative Genomics Viewer (IGV) for visualization. Genome_build: TAIR10 Supplementary_files_format_and_content: bed files
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Submission date |
Dec 26, 2019 |
Last update date |
Jun 21, 2020 |
Contact name |
yaoguang yu |
Organization name |
SUN YAT-SEN UNIVERSITY
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Street address |
No. 135, Xingang Xi Road
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City |
Guangzhou |
ZIP/Postal code |
510275 |
Country |
China |
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Platform ID |
GPL26208 |
Series (2) |
GSE142369 |
BRIP1/2 are core members of SWI/SNF complex |
GSE142621 |
Genome-wide profiling of SWI/SNF chromatin remodeler BRAHMA (BRM) and BRM Interacting Protein 1/2 (BRIP1/2) in Arabidopsis |
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Relations |
BioSample |
SAMN13683229 |
SRA |
SRX7442904 |
Supplementary file |
Size |
Download |
File type/resource |
GSM4233037_brip1brip2_BRM-GFP-IP-bio1_peaks.bed.gz |
139.3 Kb |
(ftp)(http) |
BED |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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