|
| Status |
Public on Jan 30, 2020 |
| Title |
MAX_affinity |
| Sample type |
SRA |
| |
|
| Source name |
Synthetic library designed based on yeast promoters
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: BY4743
tissue: Synthetic library designed based on yeast promoters
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
After TF directed transposition induction and selection, plasmid DNA is extracted using Qiagen mini prep kit
We amplify all TF directed transpositions from library plasmids DNA by PCR
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Library strategy: Calling Cards Reporter Arrays
We use our python analysis source code for data processing. The code is available on Gitlab
Genome_build: not applicable
Supplementary_files_format_and_content: The processed data files is in CSV format each row as a synthetic promoter sequence. Columns contain transposition insertion information and read count information.
|
| |
|
| Submission date |
Jan 29, 2020 |
| Last update date |
Feb 01, 2020 |
| Contact name |
Jiayue Liu |
| E-mail(s) |
jiayue@wustl.edu
|
| Organization name |
Washington University in St. Louis
|
| Department |
Genetics
|
| Lab |
Mitra
|
| Street address |
4515 McKinley Research Building
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63108 |
| Country |
USA |
| |
|
| Platform ID |
GPL19756 |
| Series (1) |
| GSE144437 |
Transcription factor binding on designed libraries measured by CCRAs |
|
| Relations |
| BioSample |
SAMN13939930 |
| SRA |
SRX7646041 |
