|
| Status |
Public on Aug 23, 2020 |
| Title |
C12_bld_rep5 |
| Sample type |
RNA |
| |
|
| Source name |
C12 bld rep5
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6129SF2 WT
tissue: blood
age: 12 weeks
|
| Treatment protocol |
Mice at 12 or 52 w.o.a. were anesthetized and killed by decapitation. Hippocampi were immediately stored, and bloods were collected in RNeasy Protect Animal Blood Tube (QIAGEN, #73224) and stored at -80℃ for RNA processing.
|
| Growth protocol |
The 3xTg-AD male mice (n=8 at 12 w.o.a; n=8 at 52 w.o.a) and age-matched B6129SF2 WT male mice (n=8 at 12 w.o.a; n=8 at 52 w.o.a) were bred at Ehime University from parents originally purchased from Jackson Laboratory (Bar Harbor, ME, USA, 3xTG-AD, MMRRC #34830; B6129SF1/J, JAX #101043) in a specific pathogen-free facility under climate-controlled conditions at room temperature (22 +/- 2 ℃) with 50% humidity and a 12-h light/dark cycle. Mice were provided with water and standard diet (MF, Oriental Yeast Co. Ltd.) at libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from frozen hippocampi using RNeasy Mini Kit (QIAGEN, #74104) and from blood using RNeasy Protect Animal Blood Kit (QIAGEN, #73224) according to the manufacturer instructions.
|
| Label |
Cy3
|
| Label protocol |
RNA (50ng) was used for microarray analysis. The Low Input Quick Amp Labeling Kit (Agilent) was used to generate amplified and biotinylated sense-strand DNA targets.
|
| |
|
| Hybridization protocol |
Hybridization, washing and scanning steps with SurePrint G3 Mouse 8x60K ver.2.0 were conducted using Gene Expression Hybridization Kit (Agilent), Gene Expression Wash Pack (Agilent, #5188-5327) and Agilent Microarray Scanner (G2505C).
|
| Scan protocol |
Hybridization, washing and scanning steps with SurePrint G3 Mouse 8x60K ver.2.0 were conducted using Gene Expression Hybridization Kit (Agilent), Gene Expression Wash Pack (Agilent, #5188-5327) and Agilent Microarray Scanner (G2505C).
|
| Description |
Blood gene expression at 12 week
|
| Data processing |
The 75th percentile shift normalization and baseline transformation(baseline to median of all samples (all bloods or all hippocampi)) were performed using Agilent GeneSpring GX ver 14.9.
|
| |
|
| Submission date |
Jan 29, 2020 |
| Last update date |
Aug 24, 2020 |
| Contact name |
Junichi Iga |
| E-mail(s) |
igajunichi@hotmail.com
|
| Organization name |
Ehime University
|
| Department |
Neuropsychiatry
|
| Street address |
Shitsukawa 454
|
| City |
Toon |
| State/province |
Ehime |
| ZIP/Postal code |
7910295 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21163 |
| Series (1) |
| GSE144459 |
Identifying blood transcriptome biomarkers of Alzheimer’s disease using transgenic mice |
|
