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Status |
Public on Aug 23, 2020 |
Title |
AD52_Hip_rep7 |
Sample type |
RNA |
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Source name |
AD52 Hip rep7
|
Organism |
Mus musculus |
Characteristics |
strain: 3xTg-AD tissue: hippocampus age: 52 weeks
|
Treatment protocol |
Mice at 12 or 52 w.o.a. were anesthetized and killed by decapitation. Hippocampi were immediately stored, and bloods were collected in RNeasy Protect Animal Blood Tube (QIAGEN, #73224) and stored at -80℃ for RNA processing.
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Growth protocol |
The 3xTg-AD male mice (n=8 at 12 w.o.a; n=8 at 52 w.o.a) and age-matched B6129SF2 WT male mice (n=8 at 12 w.o.a; n=8 at 52 w.o.a) were bred at Ehime University from parents originally purchased from Jackson Laboratory (Bar Harbor, ME, USA, 3xTG-AD, MMRRC #34830; B6129SF1/J, JAX #101043) in a specific pathogen-free facility under climate-controlled conditions at room temperature (22 +/- 2 ℃) with 50% humidity and a 12-h light/dark cycle. Mice were provided with water and standard diet (MF, Oriental Yeast Co. Ltd.) at libitum.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from frozen hippocampi using RNeasy Mini Kit (QIAGEN, #74104) and from blood using RNeasy Protect Animal Blood Kit (QIAGEN, #73224) according to the manufacturer instructions.
|
Label |
Cy3
|
Label protocol |
RNA (50ng) was used for microarray analysis. The Low Input Quick Amp Labeling Kit (Agilent) was used to generate amplified and biotinylated sense-strand DNA targets.
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Hybridization protocol |
Hybridization, washing and scanning steps with SurePrint G3 Mouse 8x60K ver.2.0 were conducted using Gene Expression Hybridization Kit (Agilent), Gene Expression Wash Pack (Agilent, #5188-5327) and Agilent Microarray Scanner (G2505C).
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Scan protocol |
Hybridization, washing and scanning steps with SurePrint G3 Mouse 8x60K ver.2.0 were conducted using Gene Expression Hybridization Kit (Agilent), Gene Expression Wash Pack (Agilent, #5188-5327) and Agilent Microarray Scanner (G2505C).
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Description |
Hippocampal gene expression at 52 week
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Data processing |
The 75th percentile shift normalization and baseline transformation(baseline to median of all samples (all bloods or all hippocampi)) were performed using Agilent GeneSpring GX ver 14.9.
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Submission date |
Jan 29, 2020 |
Last update date |
Aug 24, 2020 |
Contact name |
Junichi Iga |
E-mail(s) |
igajunichi@hotmail.com
|
Organization name |
Ehime University
|
Department |
Neuropsychiatry
|
Street address |
Shitsukawa 454
|
City |
Toon |
State/province |
Ehime |
ZIP/Postal code |
7910295 |
Country |
Japan |
|
|
Platform ID |
GPL21163 |
Series (1) |
GSE144459 |
Identifying blood transcriptome biomarkers of Alzheimer’s disease using transgenic mice |
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