|
| Status |
Public on Sep 20, 2009 |
| Title |
mst_wt_rep2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
wild type, rep 2
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
cell line: Hy368 (h-, leu1-32, ura4-D18, ade6-M210)
strain: wt
treatment: none
|
| Growth protocol |
Strain was grown on YES medium at 25C to log phase
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
See reference: Xue, Y. et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 21, 25-39 (2004).
|
| Label |
Cy5
|
| Label protocol |
See reference: Xue, Y. et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 21, 25-39 (2004).
|
| |
|
| Channel 2 |
| Source name |
mutant ∆mst, rep 2
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
cell line: Hu0988 (h-, mst2::URA4, leu1-32, ura4-D18, ade6-M210)
strain: mst
treatment: none
|
| Growth protocol |
Strain was grown on YES medium at 25C to log phase
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
See reference: Xue, Y. et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 21, 25-39 (2004).
|
| Label |
Cy3
|
| Label protocol |
See reference: Xue, Y. et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 21, 25-39 (2004).
|
| |
|
| |
| Hybridization protocol |
See reference: Xue, Y. et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 21, 25-39 (2004).
|
| Scan protocol |
Slides were scanned using Bio-rad Vers-Array ChipReader. Scanned slides were quantified with Imagene 4.2 software.
|
| Description |
mst_wt_rep2
|
| Data processing |
Quantified data were loaded to GeneSpring software and normalized by lowess normalization
|
| |
|
| Submission date |
Jul 22, 2009 |
| Last update date |
Jul 27, 2009 |
| Contact name |
Susan Forsburg |
| E-mail(s) |
forsburg@usc.edu
|
| Phone |
213-740-7342
|
| Fax |
213-740-8631
|
| URL |
http://www.pombe.net
|
| Organization name |
University of Southern California
|
| Department |
Molecular and Computational Biology
|
| Lab |
Forsburg
|
| Street address |
1050 Childs Way, RRI 201B
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90089-2910 |
| Country |
USA |
| |
|
| Platform ID |
GPL7734 |
| Series (2) |
| GSE17259 |
S. pombe acetyltransferase mutants identifies redundant pathways of gene regulation, dual-channel dataset |
| GSE17298 |
Expression profiling of S. pombe acetyltransferase mutants identifies redundant pathways of gene regulation |
|
