|
Status |
Public on Sep 20, 2009 |
Title |
mst_wt_rep2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
wild type, rep 2
|
Organism |
Schizosaccharomyces pombe |
Characteristics |
cell line: Hy368 (h-, leu1-32, ura4-D18, ade6-M210) strain: wt treatment: none
|
Growth protocol |
Strain was grown on YES medium at 25C to log phase
|
Extracted molecule |
polyA RNA |
Extraction protocol |
See reference: Xue, Y. et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 21, 25-39 (2004).
|
Label |
Cy5
|
Label protocol |
See reference: Xue, Y. et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 21, 25-39 (2004).
|
|
|
Channel 2 |
Source name |
mutant ∆mst, rep 2
|
Organism |
Schizosaccharomyces pombe |
Characteristics |
cell line: Hu0988 (h-, mst2::URA4, leu1-32, ura4-D18, ade6-M210) strain: mst treatment: none
|
Growth protocol |
Strain was grown on YES medium at 25C to log phase
|
Extracted molecule |
polyA RNA |
Extraction protocol |
See reference: Xue, Y. et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 21, 25-39 (2004).
|
Label |
Cy3
|
Label protocol |
See reference: Xue, Y. et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 21, 25-39 (2004).
|
|
|
|
Hybridization protocol |
See reference: Xue, Y. et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 21, 25-39 (2004).
|
Scan protocol |
Slides were scanned using Bio-rad Vers-Array ChipReader. Scanned slides were quantified with Imagene 4.2 software.
|
Description |
mst_wt_rep2
|
Data processing |
Quantified data were loaded to GeneSpring software and normalized by lowess normalization
|
|
|
Submission date |
Jul 22, 2009 |
Last update date |
Jul 27, 2009 |
Contact name |
Susan Forsburg |
E-mail(s) |
forsburg@usc.edu
|
Phone |
213-740-7342
|
Fax |
213-740-8631
|
URL |
http://www.pombe.net
|
Organization name |
University of Southern California
|
Department |
Molecular and Computational Biology
|
Lab |
Forsburg
|
Street address |
1050 Childs Way, RRI 201B
|
City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90089-2910 |
Country |
USA |
|
|
Platform ID |
GPL7734 |
Series (2) |
GSE17259 |
S. pombe acetyltransferase mutants identifies redundant pathways of gene regulation, dual-channel dataset |
GSE17298 |
Expression profiling of S. pombe acetyltransferase mutants identifies redundant pathways of gene regulation |
|