|
| Status |
Public on Feb 12, 2024 |
| Title |
mESC RNA-rep2 |
| Sample type |
RNA |
| |
|
| Source name |
mESC RNA-rep2
|
| Organism |
Mus musculus |
| Characteristics |
cell type: embryonic stem cells
|
| Treatment protocol |
5x104 cells were seeded into plates (Nunc 4-well culture dish, Thermo Fisher Scientific, USA) coated with crosslinked ELP
|
| Growth protocol |
Cells were cultured in LIF-free ES culture medium for 2 days and then replaced with Glasgow minimum essential medium (GMEM; Gibco USA) containing 2.5% knock serum replacement (KSR, Gibco, USA), 1% NEAA and 10 μM β-ME for next 12 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated from cultured cells using PureLink RNA mini Kit (Invitrogen, Life Technologies, USA)
|
| Label |
Cy3
|
| Label protocol |
Starting with 500ng of total RNA, Cy3 labeled cRNA was produced following the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
|
| Scan protocol |
Slides were washed and then scanned with an Agilent Scanner.
|
| Description |
Gene expression for mESC
|
| Data processing |
Data was obtained using the Agilent Feature Extraction software (v12), using the 1-color defaults for all parameters.
|
| |
|
| Submission date |
Feb 20, 2020 |
| Last update date |
Feb 12, 2024 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL4134 |
| Series (1) |
| GSE145623 |
Crosslinked elastin-like polypeptides mediate direct cardiac differentiation of mouse embryonic and induced pluripotent stem cells without use of cardiac differentiation factors |
|
