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Status |
Public on Feb 12, 2024 |
Title |
Day 9 differ RNA-rep1 |
Sample type |
RNA |
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|
Source name |
Day 9 differ RNA-rep1
|
Organism |
Mus musculus |
Characteristics |
cell type: embryonic stem cells
|
Treatment protocol |
5x104 cells were seeded into plates (Nunc 4-well culture dish, Thermo Fisher Scientific, USA) coated with crosslinked ELP
|
Growth protocol |
Cells were cultured in LIF-free ES culture medium for 2 days and then replaced with Glasgow minimum essential medium (GMEM; Gibco USA) containing 2.5% knock serum replacement (KSR, Gibco, USA), 1% NEAA and 10 μM β-ME for next 12 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
total RNA was isolated from cultured cells using PureLink RNA mini Kit (Invitrogen, Life Technologies, USA)
|
Label |
Cy3
|
Label protocol |
Starting with 500ng of total RNA, Cy3 labeled cRNA was produced following the manufacturer’s protocol.
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|
|
Hybridization protocol |
For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
|
Scan protocol |
Slides were washed and then scanned with an Agilent Scanner.
|
Description |
Gene expression for Day 9
|
Data processing |
Data was obtained using the Agilent Feature Extraction software (v12), using the 1-color defaults for all parameters.
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Submission date |
Feb 20, 2020 |
Last update date |
Feb 12, 2024 |
Contact name |
NIEHS Microarray Core |
E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
Organization name |
NIEHS
|
Department |
DIR
|
Lab |
Microarray Core
|
Street address |
111 T.W. Alexander Drive
|
City |
RTP |
State/province |
NC |
ZIP/Postal code |
27709 |
Country |
USA |
|
|
Platform ID |
GPL4134 |
Series (1) |
GSE145623 |
Crosslinked elastin-like polypeptides mediate direct cardiac differentiation of mouse embryonic and induced pluripotent stem cells without use of cardiac differentiation factors |
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