|
| Status |
Public on Apr 01, 2010 |
| Title |
PTC1 with SDS and AFB1 rep1 |
| Sample type |
RNA |
| |
|
| Source name |
PTC1 with SDS and AFB1
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genetic background: BY4743
genotype: PTC1 KO
|
| Treatment protocol |
Both strains were grown in YPD medium including 0.01% SDS or 0.01% SDS with 25 ppm Aflatoxin B1.
|
| Growth protocol |
Parental 4743 (BY4743; MATa/α his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lys2Δ0) and that derivative ptc1 (PTC1 (YDL006w) knock-out strain) were grown in YPD medium at 25C for 2 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Each sample of total RNA was extracted using FastRNA Pro Red kit (MP Biomedicals, US) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Approximately 4 µg of total RNA was processed to produce biotinylated cRNA targets using Affymetrix One-Cycle Target prep protocol.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
S. cerevisiae PTC1 mutant strain grown in YPD with chemicals
Gene expression data from yeast
|
| Data processing |
CEL file MAS5 normalized
|
| |
|
| Submission date |
Jul 28, 2009 |
| Last update date |
Feb 09, 2010 |
| Contact name |
Tadahiro Suzuki |
| Organization name |
National Food Rsearch Institute
|
| Department |
Applied Microbiology Division
|
| Lab |
Microorganism Evaluation Laboratory
|
| Street address |
kan-nondai 2-1-12
|
| City |
Tukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8642 |
| Country |
Japan |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE17367 |
Yeast Genomic Expression Patterns of PTC1 null mutant in Response to Aflatoxin B1 (AFB1) |
|
