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Sample GSM435248 Query DataSets for GSM435248
Status Public on Feb 02, 2013
Title H17F_rep3_Array1
Sample type RNA
Source name whole body, 7-day-old adult, female
Organism Drosophila pseudoobscura x Drosophila persimilis
Characteristics tissue: whole body
gender: Female
age: 7-day-old
Growth protocol Flies were reared on cornmeal/yeast medium and maintained at 20°C with a 12:12-h light/dark cycle.
Extracted molecule total RNA
Extraction protocol Flies were instantly frozen in liquid nitrogen and stored in TRIZOL (Invitrogen) at -80°C. RNA was isolated using TRIZOL from at least 100 individuals. RNA quantity and quality were separately measured on a NanoDrop spectrophotometer (Thermo Scientific) and a Bioanalyzer 2100 (Agilent Technologies). High-quality samples (260/280>1.85; 260/230>1.7) further purified using the RNeasy kit (Qiagen).
Label Cy3
Label protocol Fluorescent cRNA was synthesized from 200 ng of total RNA using Agilent’s low-RNA input fluorescent linear amplification kit following manufacturer's protocols (Agilent Technologies). Labelled RNAs were cleaned with RNeasy columns (Qiagen), and cRNA yields were quantified on a NanoDrop spectrophotometer. Two separate labeling reactions per sample were pooled and hybridized to the arrays following Agilent’s protocols.
Hybridization protocol Agilent’s protocols
Scan protocol Agilent’s protocols
Description Hybrids of Drosophila pseudoobscura (maternal) and Drosophila persimilis (paternal)
Data processing Performed in HDBStat: data is normalized by flagging out bad spots, filtering unexpressed genes (average raw intensity across all the samples <50), negative and positive Agilent controls, and then normalized with Chip Mean and log2 transformation.
Submission date Jul 31, 2009
Last update date Feb 02, 2013
Contact name Carlos Alberto Machado
Organization name University of Maryland
Department department of biology
Street address 1210 BPS
City college park
State/province MD
ZIP/Postal code 20742
Country USA
Platform ID GPL8884
Series (1)
GSE17452 Aberrant gene expression in Drosophila hybrids supporting faster-male evolution

Data table header descriptions
VALUE normalized signal

Data table
GA10247_4 0.648555139
GA10247_1 1.408401711
GA10310_1PS 4.187354857
GA10310_1PER 4.648700479
GA10395_3 0.26590103
GA10395_4 0.282958288
GA10397_3 0.728312438
GA10397_1 0.753006204
GA10397_2 0.710965737
GA10397_4 0.772647139
GA10423_1PER 0.367806424
GA10423_1PS 0.397025408
GA10493_1 2.164716417
GA10493_3 1.911139975
GA10493_4 2.134674435
GA10493_2 1.811123396
GA10495_4 1.055204269
GA10495_1 1.509409518
GA10495_2 1.227753482
GA10497_4 0.527164196

Total number of rows: 25699

Table truncated, full table size 579 Kbytes.

Supplementary file Size Download File type/resource
GSM435248.gpr.gz 1.8 Mb (ftp)(http) GPR
Processed data included within Sample table

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