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| Status |
Public on Feb 02, 2013 |
| Title |
Aberrant gene expression in Drosophila hybrids supporting faster-male evolution |
| Platform organism |
Drosophila pseudoobscura |
| Sample organisms |
Drosophila pseudoobscura x Drosophila persimilis; Drosophila pseudoobscura pseudoobscura x Drosophila pseudoobscura bogotana; Drosophila persimilis x Drosophila pseudoobscura; Drosophila pseudoobscura bogotana x Drosophila pseudoobscura pseudoobscura |
| Experiment type |
Expression profiling by array
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| Summary |
To explore the aberrant expression patterns between hybrid sexes, we compare the global gene expression of 7-day-old whole body adults of hybrids by sex in recently diverged Drosophia pseudoobscura group
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| Overall design |
7-day-old virgin hybrid flies were assayed by sex. Reciprocal F1 hybrids were produced for D. pseudoobscura/D. persimilis and D. pseudoobscura/D. p. bogotana crosses by mass mating virgin flies. At least three isofemale inbred lines were used for each species (D. pseudoobscura; D. persimilis; D. pseudoobscura bogotana).Two separate labeling reactions per sample were pooled and hybridized to the Agilent single color (Cyanine 3-CTP dye) arrays. Three different replicates were hybridized for each hybrid and sex. Pure species data (collected the same time and deposited as GSE17192 ) and hybrid data were analyzed together to determine the misexpression differences between hybrid males and females.
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| Contributor(s) |
Jiang Z, Machado CA |
| Citation missing |
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| |
| Submission date |
Jul 31, 2009 |
| Last update date |
Feb 03, 2013 |
| Contact name |
Carlos Alberto Machado |
| E-mail(s) |
zjiang@umd.edu
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| Organization name |
University of Maryland
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| Department |
department of biology
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| Street address |
1210 BPS
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| City |
college park |
| State/province |
MD |
| ZIP/Postal code |
20742 |
| Country |
USA |
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| Platforms (1) |
| GPL8884 |
Drosophila pseudoobscura 25.7K |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA118721 |
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