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Sample GSM435255 Query DataSets for GSM435255
Status Public on Feb 02, 2013
Title H37M_rep1_Array3
Sample type RNA
 
Source name whole body, 7-day-old adult, female
Organism Drosophila pseudoobscura pseudoobscura x Drosophila pseudoobscura bogotana
Characteristics tissue: whole body
gender: Male
age: 7-day-old
Growth protocol Flies were reared on cornmeal/yeast medium and maintained at 20°C with a 12:12-h light/dark cycle.
Extracted molecule total RNA
Extraction protocol Flies were instantly frozen in liquid nitrogen and stored in TRIZOL (Invitrogen) at -80°C. RNA was isolated using TRIZOL from at least 100 individuals. RNA quantity and quality were separately measured on a NanoDrop spectrophotometer (Thermo Scientific) and a Bioanalyzer 2100 (Agilent Technologies). High-quality samples (260/280>1.85; 260/230>1.7) further purified using the RNeasy kit (Qiagen).
Label Cy3
Label protocol Fluorescent cRNA was synthesized from 200 ng of total RNA using Agilent’s low-RNA input fluorescent linear amplification kit following manufacturer's protocols (Agilent Technologies). Labelled RNAs were cleaned with RNeasy columns (Qiagen), and cRNA yields were quantified on a NanoDrop spectrophotometer. Two separate labeling reactions per sample were pooled and hybridized to the arrays following Agilent’s protocols.
 
Hybridization protocol Agilent’s protocols
Scan protocol Agilent’s protocols
Description Hybrids of Drosophila pseudoobscura (maternal) and Drosophila pseudoobscura bogotana (paternal)
Data processing Performed in HDBStat: data is normalized by flagging out bad spots, filtering unexpressed genes (average raw intensity across all the samples <50), negative and positive Agilent controls, and then normalized with Chip Mean and log2 transformation.
 
Submission date Jul 31, 2009
Last update date Feb 02, 2013
Contact name Carlos Alberto Machado
E-mail(s) zjiang@umd.edu
Organization name University of Maryland
Department department of biology
Street address 1210 BPS
City college park
State/province MD
ZIP/Postal code 20742
Country USA
 
Platform ID GPL8884
Series (1)
GSE17452 Aberrant gene expression in Drosophila hybrids supporting faster-male evolution

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
GA10247_4 0.805966859
GA10247_1 1.064930784
GA10310_1PS 2.836057694
GA10310_1PER 3.856335808
GA10395_3 0.677427211
GA10395_4 0.875194363
GA10397_3 0.486767064
GA10397_1 0.516693387
GA10397_2 0.476652066
GA10397_4 0.438944914
GA10423_1PER 0.511198107
GA10423_1PS 0.550304732
GA10493_1 1.1366352
GA10493_3 0.824695674
GA10493_4 0.908182923
GA10493_2 0.975176659
GA10495_4 0.64768332
GA10495_1 1.03230758
GA10495_2 0.737020647
GA10497_4 0.50457602

Total number of rows: 25699

Table truncated, full table size 580 Kbytes.




Supplementary file Size Download File type/resource
GSM435255.gpr.gz 1.8 Mb (ftp)(http) GPR
Processed data included within Sample table

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