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Sample GSM435265 Query DataSets for GSM435265
Status Public on Feb 02, 2013
Title H47F_rep2_Array1
Sample type RNA
 
Source name whole body, 7-day-old adult, male
Organism Drosophila pseudoobscura bogotana x Drosophila pseudoobscura pseudoobscura
Characteristics tissue: whole body
gender: Female
age: 7-day-old
Growth protocol Flies were reared on cornmeal/yeast medium and maintained at 20°C with a 12:12-h light/dark cycle.
Extracted molecule total RNA
Extraction protocol Flies were instantly frozen in liquid nitrogen and stored in TRIZOL (Invitrogen) at -80°C. RNA was isolated using TRIZOL from at least 100 individuals. RNA quantity and quality were separately measured on a NanoDrop spectrophotometer (Thermo Scientific) and a Bioanalyzer 2100 (Agilent Technologies). High-quality samples (260/280>1.85; 260/230>1.7) further purified using the RNeasy kit (Qiagen).
Label Cy3
Label protocol Fluorescent cRNA was synthesized from 200 ng of total RNA using Agilent’s low-RNA input fluorescent linear amplification kit following manufacturer's protocols (Agilent Technologies). Labelled RNAs were cleaned with RNeasy columns (Qiagen), and cRNA yields were quantified on a NanoDrop spectrophotometer. Two separate labeling reactions per sample were pooled and hybridized to the arrays following Agilent’s protocols.
 
Hybridization protocol Agilent’s protocols
Scan protocol Agilent’s protocols
Description Hybrids of Drosophila pseudoobscura (paternal) and Drosophila pseudoobscura bogotana (maternal)
Data processing Performed in HDBStat: data is normalized by flagging out bad spots, filtering unexpressed genes (average raw intensity across all the samples <50), negative and positive Agilent controls, and then normalized with Chip Mean and log2 transformation.
 
Submission date Jul 31, 2009
Last update date Feb 02, 2013
Contact name Carlos Alberto Machado
E-mail(s) zjiang@umd.edu
Organization name University of Maryland
Department department of biology
Street address 1210 BPS
City college park
State/province MD
ZIP/Postal code 20742
Country USA
 
Platform ID GPL8884
Series (1)
GSE17452 Aberrant gene expression in Drosophila hybrids supporting faster-male evolution

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
GA10247_4 0.716397795
GA10247_1 1.503050544
GA10310_1PS 4.205421426
GA10310_1PER 4.5944566
GA10395_3 0.271875288
GA10395_4 0.299950713
GA10397_3 0.788430411
GA10397_1 0.787228818
GA10397_2 0.754401641
GA10397_4 0.80574197
GA10423_1PER 0.569667197
GA10423_1PS 0.656459589
GA10493_1 2.106158887
GA10493_3 1.858952657
GA10493_4 2.034037576
GA10493_2 1.748145618
GA10495_4 1.206721494
GA10495_1 1.770511984
GA10495_2 1.253587826
GA10497_4 0.547837689

Total number of rows: 25699

Table truncated, full table size 580 Kbytes.




Supplementary file Size Download File type/resource
GSM435265.gpr.gz 1.8 Mb (ftp)(http) GPR
Processed data included within Sample table

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