|
| Status |
Public on Aug 06, 2009 |
| Title |
Global gene expression of NASH patients, normal control A643 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA purchased from ADMET Technologies (Durham, NC), extracted from healthy liver tissue.
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: normal (control)
tissue: liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples were stored in RNAlater at -80 freezer before total RNA extraction with Qiagen RNeasy kit.
|
| Label |
Biotin
|
| Label protocol |
Complementary DNA was prepared from total RNA.
In vitro transcription was then performed with biotin labeled substrate to produce biotin labeled cRNA.
|
| |
|
| Hybridization protocol |
1, Fragmentation of cRNA.
2, Denature cRNA at 90C for 5 min.
3, Hybridization was performed at 37 C, for 18-24 hours.
4, Detection with Cy5-streptavidin conjugate.
|
| Scan protocol |
Microarray scanning and data acquisition were done at the gene expression facility, Roswell Park Cancer Institute, following their routine standard procedure.
|
| Description |
These samples were derived from liver grafts of normal subjects with normal body mass index. The ages of these normal controls were matched with our NASH patients. No liver diseases were diagnosed with these normal controls. Although the biochemistry data provided by Admet were limited, the healthy status of these livers was ascertained by the lower transcription levels of marker genes for inflammation and fibrosis.
|
| Data processing |
Data were median-normalized for comparison. Medium normalization were done by our collaborators at Roswell Park cancer institute and verified using MS Excel2003.
|
| |
|
| Submission date |
Aug 02, 2009 |
| Last update date |
Oct 20, 2010 |
| Contact name |
Susan Baker |
| E-mail(s) |
sbaker@upa.chob.edu, lixinzhu@buffalo.edu
|
| Organization name |
SUNY at Buffalo
|
| Department |
Pediatrics
|
| Lab |
Digestive Diseases and Nutrition Center Research Laboratory
|
| Street address |
3435 Main street, 422 BRB
|
| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14214 |
| Country |
USA |
| |
|
| Platform ID |
GPL2895 |
| Series (2) |
| GSE17470 |
Global gene expression of NASH patients |
| GSE24807 |
Increased hemoglobin expression in NASH livers |
|
