|
| Status |
Public on Apr 27, 2020 |
| Title |
E8.5 WT |
| Sample type |
RNA |
| |
|
| Source name |
Wild type
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: Wild type
age: E8.5
tissue: whole embryo
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was isolated from cells using TRIzol Reagent (nitrogen) and purified using SV Total RNA Isolation System (Promega) according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
cRNA was amplified and labelled using a Low input Quick Amp Labelling Kit (Agilent Technologies).
|
| |
|
| Hybridization protocol |
cRNA was hybridized to a 60K 60-mer oligomicroarray (SurePrint G3 Mouse Gene Expression Microarray 8x60K v2 ; Agilent Technologies) according to the manufacturer's instructions.
|
| Scan protocol |
The hybridized microarray slides were scanned using an Agilent scanner. The relative hybridization intensities and background hybridization values were calculated using Feature Extraction Software version 9.5.1.1 (Agilent Technologies).
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.5.1.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. The raw signal intensities and flags for each probe were calculated from the hybridization intensities and spot information according to the procedures recommended by Agilent Technologies using the Flag criteria in the GeneSpring Software. In addition, the raw signal intensities of all samples were normalized by the quantile algorithm with the Bioconductor.
quantile normalized signal (non-log scaled) and ABS CALL.
|
| |
|
| Submission date |
Mar 09, 2020 |
| Last update date |
Apr 29, 2020 |
| Contact name |
Satomi Yogosawa |
| Organization name |
Jikei University School of Medicine
|
| Department |
Department of Biochemistry
|
| Street address |
3-25-8, Nishi-shinbasi
|
| City |
Minato-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
105-8461 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21163 |
| Series (1) |
| GSE146614 |
DYRK2 knockout mice exhibit fetal growth retardation |
|
