|
| Status |
Public on Mar 01, 2010 |
| Title |
4,7hpf MZ replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
MZ whole embryo
|
| Organism |
Danio rerio |
| Characteristics |
hours post fertilization: 4.7
cycloheximide treatment at 1,5 hpf: NO
microinjection: NO
|
| Treatment protocol |
Embryos of the genotypes indicated were injected with 100 pg/embryo Pou5f1 mRNA, 50 pg/embryo Sox2 mRNA or left uninjected. At 1,5 hpf, the embryos were placed into 15 mkg/ml Cycloheximide solution (Calbiochem), until the embryos were frozen for RNA isolation
|
| Growth protocol |
, WT embryos of AB x TÜB strain crosses (www.ZFIN.org) and MZ embryos carrying the m793 allele of the spg mutation were used
|
| Extracted molecule |
total RNA |
| Extraction protocol |
60-100 embryos per sample were snap-frozen in liquid nitrogen, and total RNA was isolated using the RNA Easy kit (Qiagen). Sample quality was assessed in an Agilent Bioanalyzer 2100, using the RNA 6000 nano Assay Kit
|
| Label |
Cy5
|
| Label protocol |
Samples were processed by Agilent Technologies Two-Color Microarray-Based Gene Expression Analysis kit according to Agilent Two-color Microarray based gene expression analysis protocoll
|
| |
|
| Hybridization protocol |
Agilent Gene Expression Hybridization Kit was used . Micorarrays were hybridized in the Agilent 2545A hybridization oven according Agilent Two-color Microarray based gene expression analysis protocoll
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner
|
| Description |
4,7hpf__MZ_replicate 3
|
| Data processing |
Data were extracted using Agilent Feature Extraction Software and GE2-v5_95_Feb07 protocoll.
|
| |
|
| Submission date |
Aug 14, 2009 |
| Last update date |
Feb 06, 2023 |
| Contact name |
Daria Onichtchouk |
| E-mail(s) |
daria.onichtchouk@biologie.uni-freiburg.de
|
| Phone |
00497612032595
|
| Organization name |
University of Freiburg
|
| Street address |
Hauptstrasse 1
|
| City |
Freiburg |
| ZIP/Postal code |
79104 |
| Country |
Germany |
| |
|
| Platform ID |
GPL7302 |
| Series (2) |
| GSE17659 |
Experiment 2: Pou5f1 and Sox2 overexpression experiments with or without protein synthesis inhibition |
| GSE17667 |
Pou5f1 transcription targets in zebrafish |
|
