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Sample GSM442036 Query DataSets for GSM442036
Status Public on Aug 20, 2009
Title A549 lung adenocarcinoma cell line treated with TGFB1 protein for 2 h, experiment #2
Sample type RNA
 
Source name Human A549 lung adenocarcinoma cell line, obtained from the American type Culture Collection (Manassas, VA).
Organism Homo sapiens
Characteristics cell line: A549
Treatment protocol Cells were serum-starved for 24 h and then treated with 5 ng/mL porcine transforming growth factor beta 1 (TGF-beta, R&D Systems, Minneapolis, MN) for indicated times.
Growth protocol A549 cells were maintained in RPMI-1640 medium with glutamine, supplemented with 10% FBS, penicillin, and streptomycin and tested for mycoplasma contamination. All tissue culture media and media supplements were purchased from Life Technologies (Gaithersburg, MD).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the harvested cells using Trizol (Invitrogen) and cleanup performed with an RNeasy kit (Qiagen)
Label biotin
Label protocol Biotinylated cRNA was prepare from 3-5 ug total RNA using standard Affymetrix protocol
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description A549 lung adenocarcinoma cell line treated with TGFB1 protein for 2 h, experiment #2
Data processing Ann Arbor quantile-normalized trimmed-mean method (PMID: 15705192).
 
Submission date Aug 18, 2009
Last update date Aug 20, 2009
Contact name Rork Kuick
E-mail(s) rork@umich.edu
Phone 734-936-9241
Organization name University of Michigan
Street address University of Michigan, SPH II, 1415 Washington Heights, Room M2533
City Ann Arbor
State/province MI
ZIP/Postal code 48109-2029
Country USA
 
Platform ID GPL570
Series (1)
GSE17708 Time Course of TGF-beta treatment of A549 lung adenocarcinoma cell line

Data table header descriptions
ID_REF
VALUE quantile-normalized trimmed-mean, log-transformed with log (max(x+50,0) + 50) using base 10 logarithms

Data table
ID_REF VALUE
1007_s_at 3.873611197
1053_at 3.081347308
117_at 2.303196057
121_at 3.063708559
1255_g_at 2.190331698
1294_at 2.767155866
1316_at 2.507855872
1320_at 2.330413773
1405_i_at 2.093421685
1431_at 2.117271296
1438_at 2.46686762
1487_at 3.579440597
1494_f_at 2.592176757
1552256_a_at 3.570309385
1552257_a_at 3.186673867
1552258_at 2.389166084
1552261_at 2.403120521
1552263_at 2.474216264
1552264_a_at 2.84509804
1552266_at 2.235528447

Total number of rows: 54675

Table truncated, full table size 1210 Kbytes.




Supplementary file Size Download File type/resource
GSM442036_Venkat_012.CEL.gz 8.2 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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