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| Status |
Public on Aug 20, 2009 |
| Title |
Time Course of TGF-beta treatment of A549 lung adenocarcinoma cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Time Course of TGF-beta treatment of A549 lung adenocarcinoma cell line on Affymetrix HG_U133_plus_2 arrays; triplicate experiments.
The goal of the experiment is to profile temporal gene expression changes during TGF-beta-induced epithelial-mesenchymal transition (EMT). During EMT cancer cells loose their epithelial specifc proteins and gain mesenchymal proteins to acquire migratory and invasive phenotype essential for metastasis. Human A549 lung adenocarcinoma cell line was treated with 5 ng/mL TGF-beta for 0, 0.5, 1, 2, 4, 8, 16, 24, and 72 h to induce EMT. The experiment was repeated 3 times. Samples were assayed using Affymetrix HG_U133_plus_2 arrays with 54675 probe-sets, using standard techniques. We provide the raw .CEL files and a supplementary Excel spreadsheet with log-transformed data and selected results from a statistical analysis.
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| Overall design |
Human A549 lung adenocarcinoma cell line was treated with 5 ng/mL TGF-beta for 0, 0.5, 1, 2, 4, 8, 16, 24, and 72 h. The experiment was repeated 3 times. Samples were assayed using Affymetrix HG_U133_plus_2 arrays with 54675 probe-sets, using standard techniques. The 2 h sample of the third experiment was not run on an array due to poor RNA, so that only 26 arrays were run.
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| Contributor(s) |
Keshamouni VG, Sartor MA, Mahvisno V, Cavalcoli J, Wright Z, Karnovsky A, Kuick R, Jagadish HV, Mirel B, Weymouth T, Athey B, Omenn GS |
| Citation(s) |
20007254 |
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| Submission date |
Aug 18, 2009 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Rork Kuick |
| E-mail(s) |
rork@umich.edu
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| Phone |
734-936-9241
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| Organization name |
University of Michigan
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| Street address |
University of Michigan, SPH II, 1415 Washington Heights, Room M2533
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| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109-2029 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (26)
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| GSM442026 |
A549 lung adenocarcinoma cell line, untreated, experiment #1 |
| GSM442027 |
A549 lung adenocarcinoma cell line, untreated, experiment #2 |
| GSM442028 |
A549 lung adenocarcinoma cell line, untreated, experiment #3 |
| GSM442029 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 0.5 h, experiment #1 |
| GSM442030 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 0.5 h, experiment #2 |
| GSM442031 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 0.5 h, experiment #3 |
| GSM442032 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 1 h, experiment #1 |
| GSM442033 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 1 h, experiment #2 |
| GSM442034 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 1 h, experiment #3 |
| GSM442035 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 2 h, experiment #1 |
| GSM442036 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 2 h, experiment #2 |
| GSM442037 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 4 h, experiment #1 |
| GSM442038 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 4 h, experiment #2 |
| GSM442039 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 4 h, experiment #3 |
| GSM442040 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 8 h, experiment #1 |
| GSM442041 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 8 h, experiment #2 |
| GSM442042 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 8 h, experiment #3 |
| GSM442043 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 16 h, experiment #1 |
| GSM442044 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 16 h, experiment #2 |
| GSM442045 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 16 h, experiment #3 |
| GSM442046 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 24 h, experiment #1 |
| GSM442047 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 24 h, experiment #2 |
| GSM442048 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 24 h, experiment #3 |
| GSM442049 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 72 h, experiment #1 |
| GSM442050 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 72 h, experiment #2 |
| GSM442051 |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 72 h, experiment #3 |
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| Relations |
| BioProject |
PRJNA118457 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE17708_Keshamouni_TGFB1_logs.xls.gz |
21.3 Mb |
(ftp)(http) |
XLS |
| GSE17708_RAW.tar |
217.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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