|
| Status |
Public on Aug 20, 2009 |
| Title |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 4 h, experiment #2 |
| Sample type |
RNA |
| |
|
| Source name |
Human A549 lung adenocarcinoma cell line, obtained from the American type Culture Collection (Manassas, VA).
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
|
| Treatment protocol |
Cells were serum-starved for 24 h and then treated with 5 ng/mL porcine transforming growth factor beta 1 (TGF-beta, R&D Systems, Minneapolis, MN) for indicated times.
|
| Growth protocol |
A549 cells were maintained in RPMI-1640 medium with glutamine, supplemented with 10% FBS, penicillin, and streptomycin and tested for mycoplasma contamination. All tissue culture media and media supplements were purchased from Life Technologies (Gaithersburg, MD).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the harvested cells using Trizol (Invitrogen) and cleanup performed with an RNeasy kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepare from 3-5 ug total RNA using standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
A549 lung adenocarcinoma cell line treated with TGFB1 protein for 4 h, experiment #2
|
| Data processing |
Ann Arbor quantile-normalized trimmed-mean method (PMID: 15705192).
|
| |
|
| Submission date |
Aug 18, 2009 |
| Last update date |
Aug 20, 2009 |
| Contact name |
Rork Kuick |
| E-mail(s) |
rork@umich.edu
|
| Phone |
734-936-9241
|
| Organization name |
University of Michigan
|
| Street address |
University of Michigan, SPH II, 1415 Washington Heights, Room M2533
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109-2029 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE17708 |
Time Course of TGF-beta treatment of A549 lung adenocarcinoma cell line |
|
