|
| Status |
Public on Mar 25, 2020 |
| Title |
1w_2 |
| Sample type |
RNA |
| |
|
| Source name |
1 weeks after vocal fold injury
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: vocal fold
strain: Sprague-Dawley
treatment: 1 weeks after vocal fold injury
|
| Treatment protocol |
The vocal fold was injured by separating and removing the lamina propria from the thyroarytenoid muscle.
|
| Growth protocol |
All mice were kept at least 1 wk at 22°C and 55% relative humidity in a 12-h day/night lighting environment with free access to food and water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For microarray profiling, the rat vocal fold total RNA was harvested using TRIzol reagent (Invitrogen, Carlsbad, Canada) and purified using RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. The amount and quality of RNA were determined using UV-Vis Spectrophotometer (Thermo, NanoDrop 2000, USA) at the absorbance of 260 nm. The mRNA expression profiling was measured using Affymetrix ClariomTM D mouse Array (Affymetrix GeneChip, Santa Clara CA, USA), which contains 65957 gene-level probe sets.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-8 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10ug of cRNA were hybridized for 16 hr at 45℃ on [miRNA-4] Affymetrix Multispecies miRNA-4 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Genechips were scanned by GeneChip® Scanner 3000.
|
| Data processing |
The data were analyzed with Expression Console™, Version 1.3.1.
|
| |
|
| Submission date |
Mar 24, 2020 |
| Last update date |
Mar 25, 2020 |
| Contact name |
李 玉琳 |
| Organization name |
Beijing Institute of Heart Lung and Blood Vessel Diseases
|
| Street address |
2 Anzhen Rd, Chaoyang District,
|
| City |
Beijing |
| ZIP/Postal code |
100029 |
| Country |
China |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE147473 |
Expression data from rat vocal fold at different periods after vocal fold injury (miRNA) |
|
