|
| Status |
Public on Mar 22, 2010 |
| Title |
α-amantin-treated BCB two-cell embryos, Sample2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse two-cell embryos, BCB, α-amantin-treated
|
| Organism |
Mus musculus |
| Characteristics |
strain of maternal pronucleus: C3H
strain of paternal pronucleus: B6
strain of ooplasm: B6
developmental stage: two-cell embryos
treatment: α-amantin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Picopure RNA extraction kit.
|
| Label |
biotin
|
| Label protocol |
ENZO RNA labelling kit.
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures.
|
| Scan protocol |
Standard Affymetrix procedures.
|
| Description |
BCB+a2
|
| Data processing |
Microarray Analysis Suite 5.0 (MAS, Affymetrix) was used to quantify the microarray signals with default analysis parameters and global scaling to target a mean equal to 150 signal units.
|
| |
|
| Submission date |
Aug 31, 2009 |
| Last update date |
Mar 22, 2010 |
| Contact name |
Zhiming Han |
| E-mail(s) |
hanzm@temple.edu
|
| Phone |
2157078458
|
| Organization name |
Temple University
|
| Department |
Fels Institute for Cancer Research and Molecular Biology
|
| Street address |
3307 North Broad Street, AHB 561
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19140 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE17886 |
Gene expression data of BBB and BCB two-cell embryos |
|
