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| Status |
Public on Mar 22, 2010 |
| Title |
Gene expression data of BBB and BCB two-cell embryos |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We constructed one-cell stage embryos by maternal pronuclear (mPN) transfer having B6 ooplasm, B6 paternal PN (pPN), and either B6 or C3H mPN (BBB and BCB, respectively). We collected embryos of each type that were either treated (BBB+a, BCB+a) or untreated with α-amanitin (BBB, BCB) at the two-cell stage for microarray analysis.
Comparison of the transcriptomes of these different kinds of embryos revealed genes for which expression differs according to maternal PN strain of origin, and the α-amanitin data revealed which of these differences is due to gene transcription, as opposed to any transcription-independent differences attributable to ooplasm-derived maternal mRNA pools.
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| Overall design |
There are 4 replicates for each kind/treatment two-cell embryos (BBB, BCB, BBB+a, BCB+a).
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| Contributor(s) |
Han Z, Mtango NR, Zhong Z, Vassena R, Latham KE |
| Citation(s) |
20107036 |
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| Submission date |
Aug 31, 2009 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Zhiming Han |
| E-mail(s) |
hanzm@temple.edu
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| Phone |
2157078458
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| Organization name |
Temple University
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| Department |
Fels Institute for Cancer Research and Molecular Biology
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| Street address |
3307 North Broad Street, AHB 561
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19140 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (16)
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| GSM446702 |
BBB two-cell embryos, Sample4 |
| GSM446703 |
BCB two-cell embryos, Sample1 |
| GSM446704 |
BCB two-cell embryos, Sample2 |
| GSM446705 |
BCB two-cell embryos, Sample3 |
| GSM446706 |
BCB two-cell embryos, Sample4 |
| GSM446707 |
α-amantin-treated BBB two-cell embryos, Sample1 |
| GSM446708 |
α-amantin-treated BBB two-cell embryos, Sample2 |
| GSM446709 |
α-amantin-treated BBB two-cell embryos, Sample3 |
| GSM446710 |
α-amantin-treated BBB two-cell embryos, Sample4 |
| GSM446711 |
α-amantin-treated BCB two-cell embryos, Sample1 |
| GSM446712 |
α-amantin-treated BCB two-cell embryos, Sample2 |
| GSM446713 |
α-amantin-treated BCB two-cell embryos, Sample3 |
| GSM446714 |
α-amantin-treated BCB two-cell embryos, Sample4 |
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| Relations |
| BioProject |
PRJNA120069 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE17886_RAW.tar |
60.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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