|
| Status |
Public on Sep 24, 2021 |
| Title |
CB74_9_4 |
| Sample type |
SRA |
| |
|
| Source name |
Kidney Graft
|
| Organisms |
Homo sapiens; Mus musculus |
| Characteristics |
cell type: hiPSC derived thymic epithelial progenitors
|
| Growth protocol |
For differentiation, iPSCs were plated on Matrigel at 3.15e5 cells/cm2. Differentiations were initiated 24 hours after plating and were carried out in X-VIVO10 (Lonza 04-743Q). Factors were added at the following concentrations: Activin A, 100ng/ml (d0-4); Wnt3a, 50ng/ml (d0, and d9-13); TTNPB (RAR agonist) 6nM (d4-13); BMP4, 20ng/ml (d5-13); LY364947, 5M (d5-13); FGF8b or FGF8a, 50ng/ml (d9-13); SAG, 100ng/ml (d5-8); SANT-1, 0.25M (d9-13) insulin-transferrin-selenium, 1:5,000 (d0) or 1;2,000 (d1-4). At day 20, cultures where transplated into athymic nude mice under the kidney capsule. Grafts were harvested 14-17 post transplantation and prepared for either bulk or scRNA-seq.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen RNeasy Minikit (Cat No./ID: 74106). RNA was then submitted to the Genomics and Microarray Shared Resource Core at the University of Colorado Anschutz Medical Campus
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
scRNA-seq
|
| Data processing |
none provided by the submitter
Supplementary_files_format_and_content: RPKM (Bulk)
Supplementary_files_format_and_content: barcodes (single cells)
Supplementary_files_format_and_content: features (single cells)
Supplementary_files_format_and_content: matrix (single cells)
|
| |
|
| Submission date |
Apr 10, 2020 |
| Last update date |
Sep 24, 2021 |
| Contact name |
Holger A Russ |
| E-mail(s) |
HOLGER.RUSS@CUANSCHUTZ.EDU
|
| Organization name |
University of Colorado Anschutz Medical Campus
|
| Department |
Barbara Davis Center for Diabetes
|
| Street address |
1775 Aurora Ct
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platform ID |
GPL25526 |
| Series (1) |
| GSE148495 |
Generation of a functional, patient specific human thymus from induced pluripotent stem cell in vivo |
|
| Relations |
| BioSample |
SAMN14575460 |
| SRA |
SRX8109371 |
