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| Status |
Public on Mar 19, 2021 |
| Title |
Cow: 4381 Trt: 0 |
| Sample type |
SRA |
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| Source name |
Milk RNA
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| Organism |
Bos taurus |
| Characteristics |
breed: Holstein
stage of lactation: Early Lactation
treatment: control
sample type: milk
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| Extracted molecule |
total RNA |
| Extraction protocol |
Milk samples were collected and centrifuged at 2,000 x g for 10 min to separate the milk fat. The milk fat was removed to a separate vial, an equal volume of RNAse free water and Trizol added. An equal volume of chloroform was added and the entire sample was centrifuged at 12,000 x g. After centrifuging 70% ethanol was added. RNA was cleaned by Qiagen RNAeasy Mini Kit.
RNA libraries were prepared for sequencing using standard Illumina protocols
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Description |
cow4381B2P4L0
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| Data processing |
Reads were mapped to the ENSEMBL bovine genome
EBseq analysis was utilized to identify differently expressed genes
Supplementary_files_format_and_content: Fastq files followed cow number_period_sample_replicate
Supplementary_files_format_and_content: tab-delimited text file includes count values for each sample
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| Submission date |
Apr 13, 2020 |
| Last update date |
Mar 19, 2021 |
| Contact name |
Shaojun Xie |
| E-mail(s) |
xie186@purdue.edu
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| Phone |
7657140672
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| Organization name |
Purdue University
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| Street address |
3135 Decatur Street
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| City |
WEST LAFAYETTE |
| State/province |
Indiana |
| ZIP/Postal code |
47906 |
| Country |
USA |
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| Platform ID |
GPL26012 |
| Series (1) |
| GSE148587 |
Secreted milk transcriptome from early lactation dairy cows |
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| Relations |
| BioSample |
SAMN14592556 |
| SRA |
SRX8111201 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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