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Status |
Public on Mar 19, 2021 |
Title |
Cow: 4383 Trt: 63 |
Sample type |
SRA |
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Source name |
Milk RNA
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Organism |
Bos taurus |
Characteristics |
breed: Holstein stage of lactation: Early Lactation treatment: postruminal lysine treatment for 63 days sample type: milk
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Extracted molecule |
total RNA |
Extraction protocol |
Milk samples were collected and centrifuged at 2,000 x g for 10 min to separate the milk fat. The milk fat was removed to a separate vial, an equal volume of RNAse free water and Trizol added. An equal volume of chloroform was added and the entire sample was centrifuged at 12,000 x g. After centrifuging 70% ethanol was added. RNA was cleaned by Qiagen RNAeasy Mini Kit. RNA libraries were prepared for sequencing using standard Illumina protocols
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
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Description |
cow4383B1P3L63
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Data processing |
Reads were mapped to the ENSEMBL bovine genome EBseq analysis was utilized to identify differently expressed genes Supplementary_files_format_and_content: Fastq files followed cow number_period_sample_replicate Supplementary_files_format_and_content: tab-delimited text file includes count values for each sample
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Submission date |
Apr 13, 2020 |
Last update date |
Mar 19, 2021 |
Contact name |
Shaojun Xie |
E-mail(s) |
xie186@purdue.edu
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Phone |
7657140672
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Organization name |
Purdue University
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Street address |
3135 Decatur Street
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City |
WEST LAFAYETTE |
State/province |
Indiana |
ZIP/Postal code |
47906 |
Country |
USA |
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Platform ID |
GPL26012 |
Series (1) |
GSE148587 |
Secreted milk transcriptome from early lactation dairy cows |
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Relations |
BioSample |
SAMN14592553 |
SRA |
SRX8111204 |
Supplementary data files not provided |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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