|
| Status |
Public on Apr 16, 2020 |
| Title |
E_Wnt_FA withdrawal_replicate3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
E_Wnt withdrawal
|
| Organism |
Mus musculus |
| Characteristics |
cell line: E-Wnt
treatment: folic acid withdrawal
tissue: mammary tumor
|
| Growth protocol |
E-Wnt, M-Wnt and metM-Wnt cells were cultured in 10-cm plates for 72 hours using either standard RPMI 1640 medium (containing 2.2 µM folic acid) with 10% fetal bovine serum (FBS) or RPMI 1640 medium without folic acid with dialyzed FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's intructions.
|
| Label |
Cy5
|
| Label protocol |
Complementary RNA (cRNA) was generated using a Low Input Quick Amp labeling kit (Agilent, Santa Clara, CA) with NIH3T3 cells as reference RNA.
|
| |
|
| Channel 2 |
| Source name |
NIH3T3 cells
|
| Organism |
Mus musculus |
| Characteristics |
type: Common reference
|
| Growth protocol |
E-Wnt, M-Wnt and metM-Wnt cells were cultured in 10-cm plates for 72 hours using either standard RPMI 1640 medium (containing 2.2 µM folic acid) with 10% fetal bovine serum (FBS) or RPMI 1640 medium without folic acid with dialyzed FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's intructions.
|
| Label |
Cy3
|
| Label protocol |
Complementary RNA (cRNA) was generated using a Low Input Quick Amp labeling kit (Agilent, Santa Clara, CA) with NIH3T3 cells as reference RNA.
|
| |
|
| |
| Hybridization protocol |
Samples were hybridized to custom two-color 180K Agilent microarrays (BARCODE25503)
|
| Scan protocol |
Samples were scanned using an Agilent Technologies Scanner G2505C
|
| Data processing |
Data were read and processed using Agilent Feature Extraction Software (version 10.7.3.1)
|
| |
|
| Submission date |
Apr 15, 2020 |
| Last update date |
Apr 16, 2020 |
| Contact name |
michael francis coleman |
| E-mail(s) |
mcoleman@unc.edu
|
| Phone |
9199850347
|
| Organization name |
UNC
|
| Street address |
235 dauer drive
|
| City |
chapel hill |
| State/province |
North Carolina |
| ZIP/Postal code |
27516 |
| Country |
USA |
| |
|
| Platform ID |
GPL11383 |
| Series (1) |
| GSE148738 |
Effects of folic acid withdrawal on transcriptomic profiles in murine triple-negative breast cancer cell lines |
|
