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| Status |
Public on Apr 16, 2020 |
| Title |
Effects of folic acid withdrawal on transcriptomic profiles in murine triple-negative breast cancer cell lines |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We have previously shown that withdrawal of folic acid led to metabolic reprogramming and a less aggressive phenotype in a cell model of triple-negative breast cancer (TNBC). Herein, we evaluate the effects of folic acid withdrawal on transcriptomic profiles in these cells. Murine cell lines were originally derived from a pool of spontaneous mammary tumors grown in MMTV-Wnt1 transgenic mice. Based on their differential molecular characteristics and metastatic potential, these cell lines were previously characterized as non-metastatic epithelial (E-Wnt), non-metastatic mesenchymal (M-Wnt) and metastatic mesenchymal (metM-Wntliver) cells.
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| Overall design |
Using custom two-color 180K Agilent microarrays, we have determined gene expression profiles for three biological replicates of each subtype kept on standard medium (2.2 mM folic acid) or folic acid-free medium for 72 hours.
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| Contributor(s) |
Kok DE, O'Flanagan CH, Coleman MF, Ashkavand Z, Hursting SD, Krupenko SA |
| Citation |
https://doi.org/10.1016/j.biochi.2020.04.005
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| Submission date |
Apr 15, 2020 |
| Last update date |
Apr 18, 2020 |
| Contact name |
michael francis coleman |
| E-mail(s) |
mcoleman@unc.edu
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| Phone |
9199850347
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| Organization name |
UNC
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| Street address |
235 dauer drive
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| City |
chapel hill |
| State/province |
North Carolina |
| ZIP/Postal code |
27516 |
| Country |
USA |
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| Platforms (1) |
| GPL11383 |
Agi-perou-lab-custom-MM-144K-arrays-BARCODE25503 |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA625532 |
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