|
Status |
Public on Apr 16, 2020 |
Title |
metM_Wnt_FA withdrawal_replicate3 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
metM_Wnt withdrawal
|
Organism |
Mus musculus |
Characteristics |
cell line: metM-Wnt treatment: folic acid withdrawal tissue: liver metastasis
|
Growth protocol |
E-Wnt, M-Wnt and metM-Wnt cells were cultured in 10-cm plates for 72 hours using either standard RPMI 1640 medium (containing 2.2 µM folic acid) with 10% fetal bovine serum (FBS) or RPMI 1640 medium without folic acid with dialyzed FBS.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's intructions.
|
Label |
Cy5
|
Label protocol |
Complementary RNA (cRNA) was generated using a Low Input Quick Amp labeling kit (Agilent, Santa Clara, CA) with NIH3T3 cells as reference RNA.
|
|
|
Channel 2 |
Source name |
NIH3T3 cells
|
Organism |
Mus musculus |
Characteristics |
type: Common reference
|
Growth protocol |
E-Wnt, M-Wnt and metM-Wnt cells were cultured in 10-cm plates for 72 hours using either standard RPMI 1640 medium (containing 2.2 µM folic acid) with 10% fetal bovine serum (FBS) or RPMI 1640 medium without folic acid with dialyzed FBS.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's intructions.
|
Label |
Cy3
|
Label protocol |
Complementary RNA (cRNA) was generated using a Low Input Quick Amp labeling kit (Agilent, Santa Clara, CA) with NIH3T3 cells as reference RNA.
|
|
|
|
Hybridization protocol |
Samples were hybridized to custom two-color 180K Agilent microarrays (BARCODE25503)
|
Scan protocol |
Samples were scanned using an Agilent Technologies Scanner G2505C
|
Data processing |
Data were read and processed using Agilent Feature Extraction Software (version 10.7.3.1)
|
|
|
Submission date |
Apr 15, 2020 |
Last update date |
Apr 16, 2020 |
Contact name |
michael francis coleman |
E-mail(s) |
mcoleman@unc.edu
|
Phone |
9199850347
|
Organization name |
UNC
|
Street address |
235 dauer drive
|
City |
chapel hill |
State/province |
North Carolina |
ZIP/Postal code |
27516 |
Country |
USA |
|
|
Platform ID |
GPL11383 |
Series (1) |
GSE148738 |
Effects of folic acid withdrawal on transcriptomic profiles in murine triple-negative breast cancer cell lines |
|