|
| Status |
Public on Dec 31, 2021 |
| Title |
sBAT SVF_12Weeks_2 |
| Sample type |
SRA |
| |
|
| Source name |
subscapular brown adipose tissue
|
| Organism |
Oryctolagus cuniculus |
| Characteristics |
tissue: subscapular brown adipose tissue
strain: New Zealand White
age: 12 weeks
fraction: stromal vascular fraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted from total or the SVF fraction of BAT
RNA-Seq Quantification Library (Normal Library)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Description |
Rabbit.SVF.GeneExpression.FPKM.xls
|
| Data processing |
Sequencing Reads Filtering using SOAPnuke: v1.5.2
Genome Mapping using HISAT2: v2.0.4
Mapping clean reads to reference using Bowtie2: v2.2.5
Gene expression cluster analysis using Cluster v3.0
DEG detection using DEseq2
Genome build: OryCun2.0
FPKM
|
| |
|
| Submission date |
Apr 18, 2020 |
| Last update date |
Dec 31, 2021 |
| Contact name |
Hai-Bin Ruan |
| E-mail(s) |
hruan@umn.edu
|
| Phone |
6123017686
|
| Organization name |
University of Minnesota
|
| Department |
Integrative Biology and Physiology
|
| Street address |
2231 6th St SE, 3-143 CCRB
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platform ID |
GPL28431 |
| Series (1) |
| GSE148891 |
Transcriptomic reprograming during the involution of brown adipose tissue in rabbits |
|
| Relations |
| BioSample |
SAMN14616273 |
| SRA |
SRX8136135 |
