Bioytinylated cRNAs detected by fluorescently-labelled streptavidin.
Description
BJ-spt10-null cells were grown in synthetic complete medium to A600 = 0.6. RNA was prepared using an RNeasy kit (Qiagen). This is #3 of three independently prepared samples. Keywords = yeast SPT10
Data processing
Each VALUE was calculated using Affymetrix GCOS software.
Global effects of spt10 mutations on gene expression in budding yeast.
Data table header descriptions
ID_REF
Stat pairs
The number of probe pairs that are associated with a given probe set.
Stat pairs used
The number of probe pairs that were used as a basis for calculation of Value. For example, this number would reflect any probe pairs that were masked.
VALUE
An overall estimate of transcript abundance for the particular sample. Signal values are roughly proportional to the amount of transcript present.
ABS_CALL
A"call" of the presence or absence of expression of a transcript. This call indicates whether one can distinguish the signal against the background variability. P = present; M = marginal (ambiguous); A = absent.
DETECTION P-VALUE
An indicator of the statistical significance of the "present" detection call. The lower the value, the greater the significance.
