|
Status |
Public on Oct 02, 2009 |
Title |
wildtype vs cde-1, aged adults |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
cde-1 mutant aged adults
|
Organism |
Caenorhabditis elegans |
Characteristics |
strain: cde-1 tm1021 stage: aged adult tissue: gonad
|
Biomaterial provider |
Josien van Wolfswinkel
|
Treatment protocol |
250 gonads were excised and collected in Trizol LS.
|
Growth protocol |
standard C. elegans culture
|
Extracted molecule |
total RNA |
Extraction protocol |
250 gonads were excised and collected in Trizol LS.
|
Label |
Cy5
|
Label protocol |
RNA was isolated and reverse transcribed with random primers. Each cDNA sample was split and labeled with both Cy3 and Cy5.
|
|
|
Channel 2 |
Source name |
wildtype aged adults
|
Organism |
Caenorhabditis elegans |
Characteristics |
strain: N2 stage: aged adult tissue: gonad
|
Biomaterial provider |
Josien van Wolfswinkel
|
Treatment protocol |
250 gonads were excised and collected in Trizol LS.
|
Growth protocol |
standard C. elegans culture
|
Extracted molecule |
total RNA |
Extraction protocol |
250 gonads were excised and collected in Trizol LS.
|
Label |
Cy3
|
Label protocol |
RNA was isolated and reverse transcribed with random primers. Each cDNA sample was split and labeled with both Cy3 and Cy5.
|
|
|
|
Hybridization protocol |
Standard Agilent protocol
|
Scan protocol |
Standard Agilent protocol
|
Description |
NA
|
Data processing |
Feature Extraction (FE) 9.5 protocol
|
|
|
Submission date |
Sep 22, 2009 |
Last update date |
Sep 22, 2009 |
Contact name |
Rene Ketting |
E-mail(s) |
r.ketting@hubrecht.eu
|
Organization name |
Hubrecht Institute
|
Street address |
Uppsalalaan 8
|
City |
Utrecht |
ZIP/Postal code |
3584CT |
Country |
Netherlands |
|
|
Platform ID |
GPL2875 |
Series (1) |
GSE18202 |
Variation in germline gene expression in cde-1 mutant |
|