|
| Status |
Public on Sep 24, 2010 |
| Title |
MFB_3_sequence |
| Sample type |
SRA |
| |
|
| Source name |
C3H10T1/2 / MIA PaCa-2 coculture, control treated
|
| Organisms |
Homo sapiens; Mus musculus |
| Characteristics |
cell type: C3H10T1/2 / MIA PaCa-2 coculture
agent: control
|
| Treatment protocol |
control (Isl2) or 5E1 Shh-blocking antibody producing hybridoma supernatant was added to the cells during the last 24h
|
| Growth protocol |
cells were grown for 5 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Trizol, all subsequent steps were performed using Illumina's mRNA-Seq Sample Preparation Kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
non-adherent
|
| Data processing |
Alignment: Sequence reads were obtained and mapped to the human and mouse genomes using the Illumina Genome Analyzer Pipeline. eland analysis file
processed data file build: mm9 and hg18
processed data file: eland analysis file
|
| |
|
| Submission date |
Sep 24, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
henk roelink |
| E-mail(s) |
roelink@berkeley.edu
|
| Organization name |
UC Berkeley
|
| Department |
MCB
|
| Lab |
roelink
|
| Street address |
16 barker hall
|
| City |
berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720-3204 |
| Country |
USA |
| |
|
| Platform ID |
GPL10329 |
| Series (1) |
| GSE18245 |
Elucidating the stromal expression pattern in response to tumor-derived Shh |
|
| Relations |
| SRA |
SRX019205 |
| BioSample |
SAMN00011291 |
