|
Status |
Public on Sep 24, 2010 |
Title |
MFB_4_sequence |
Sample type |
SRA |
|
|
Source name |
C3H10T1/2 / MIA PaCa-2 coculture, 5E1 treated
|
Organisms |
Homo sapiens; Mus musculus |
Characteristics |
cell type: C3H10T1/2 / MIA PaCa-2 coculture agent: 5E1 blocking antibody
|
Treatment protocol |
control (Isl2) or 5E1 Shh-blocking antibody producing hybridoma supernatant was added to the cells during the last 24h
|
Growth protocol |
cells were grown for 5 days
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated using Trizol, all subsequent steps were performed using Illumina's mRNA-Seq Sample Preparation Kit
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina Genome Analyzer |
|
|
Description |
non-adherent
|
Data processing |
Alignment: Sequence reads were obtained and mapped to the human and mouse genomes using the Illumina Genome Analyzer Pipeline. eland analysis file processed data file build: mm9 and hg18 processed data file: eland analysis file
|
|
|
Submission date |
Sep 24, 2009 |
Last update date |
May 15, 2019 |
Contact name |
henk roelink |
E-mail(s) |
roelink@berkeley.edu
|
Organization name |
UC Berkeley
|
Department |
MCB
|
Lab |
roelink
|
Street address |
16 barker hall
|
City |
berkeley |
State/province |
CA |
ZIP/Postal code |
94720-3204 |
Country |
USA |
|
|
Platform ID |
GPL10329 |
Series (1) |
GSE18245 |
Elucidating the stromal expression pattern in response to tumor-derived Shh |
|
Relations |
SRA |
SRX019206 |
BioSample |
SAMN00011292 |