|
| Status |
Public on May 20, 2020 |
| Title |
Sample 5 from KC |
| Sample type |
RNA |
| |
|
| Source name |
Kupffer cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: Balb/C
tissue: Liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sorted cells from 5 animals were pooled. The total RNA was isolated from cells by using RNeasy Plus Mini Kit (Qiagen) with additional purification and concentration with RNA Clean& Concentrator (Zymo Research).
|
| Label |
n.a.
|
| Label protocol |
100 ng of total RNA was mixed with Reporter CodeSet and Capture ProbeSet from nCounter Mouse Inflammation V2 panel according to the instructions at nCounter® XT Assay User Manual (NanoString tecnology) User Manual
|
| |
|
| Hybridization protocol |
Samples were hybridized for 16 h at 65C according to the instructions at nCounter® XT Assay User Manual (NanoString tecnology)
|
| Scan protocol |
After hybridization samples were placed in the prep station of nCounter FLEX analysis system, there hybridized samples were purified and immobilized in a sample cartridge (cartridge ID 207846350819) according to standard protocol. Then the sample cartridge was scanned on the nCounter FLEX digital analyzer (Scanner ID 1410C0216)
|
| Description |
NS_Infl_Mm_v2_C2537.rlf was used as Reporter Library File
|
| Data processing |
The raw data were processed in nSolver Analysis Software 4.0. We used CodeSetContent(Housekeeping) standart normalization
|
| |
|
| Submission date |
May 19, 2020 |
| Last update date |
May 20, 2020 |
| Contact name |
Polina Vishnyakova |
| E-mail(s) |
vpa2002@mail.ru
|
| Organization name |
National Medical Research Center for Obstetrics, Gynecology and Perinatology
|
| Street address |
Oparina 4
|
| City |
Moscow |
| ZIP/Postal code |
116571 |
| Country |
Russia |
| |
|
| Platform ID |
GPL20885 |
| Series (1) |
| GSE150878 |
Differential expression of genes associated with inflammation in KC (Kupffer cells) and MNCs (blood monocytes): |
|
