|
| Status |
Public on Sep 19, 2020 |
| Title |
swarming mosquito Replicate 3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
laboratory mosquito
|
| Organism |
Anopheles gambiae |
| Characteristics |
tissue: head
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mosquitoes, mostly males, were collected in swarms using sweeping net during dusk. The indoor resting males with antennal fibrillae becoming erect were collected in inhabited houses using vacuum aspiration just prior to swarming time.
Total RNA extracted using Trizol following manufacturer's instructions, and CTAB extraction to Remove Melanin
|
| Label |
Cy5
|
| Label protocol |
Cy5-labeled control and Cy3-labeled experimental cRNA probes were generated from 200 ng of RNA using Agilent Technologies Low Input Quick Amp Labeling Kit according to the manufacturer's instructions.
|
| |
|
| Channel 2 |
| Source name |
field mosquito
|
| Organism |
Anopheles coluzzii |
| Characteristics |
tissue: head
group: swarming
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mosquitoes, mostly males, were collected in swarms using sweeping net during dusk. The indoor resting males with antennal fibrillae becoming erect were collected in inhabited houses using vacuum aspiration just prior to swarming time.
Total RNA extracted using Trizol following manufacturer's instructions, and CTAB extraction to Remove Melanin
|
| Label |
Cy3
|
| Label protocol |
Cy5-labeled control and Cy3-labeled experimental cRNA probes were generated from 200 ng of RNA using Agilent Technologies Low Input Quick Amp Labeling Kit according to the manufacturer's instructions.
|
| |
|
| |
| Hybridization protocol |
Probe hybridization to the microarray slides was performed with 2 μg cRNA probes.Hybridization buffer to stop fragmentation. Samples were applied to microarrays enclosed in A4 mixer chambers.After hybridization, slides were washed sequential
|
| Scan protocol |
Microarray slides were scanned using an Axon GenePix 4200AL scanner at 10-μm pixel size (Axon Instruments, Union City, California, USA)
The scanned images were analyzed using feature extraction software (Agilent)
|
| Description |
Biological replicate 3 of 3,swarming mosquito
|
| Data processing |
gProcessedSignal and rProcessedSignal from Agilent feature extraction output files were imported into Partek and quantile normalized and log2 transformed.
|
| |
|
| Submission date |
May 21, 2020 |
| Last update date |
Sep 21, 2020 |
| Contact name |
Sibao Wang |
| E-mail(s) |
sbwang@cemps.ac.cn
|
| Phone |
86-21-54924339
|
| Organization name |
Chinese Academy of Sciences
|
| Department |
CAS Center for Excellence in Molecular Plant Science/ Institute of Plant Physiology and Ecology
|
| Lab |
CAS key lab of insect developmental and Evolutionary Biology
|
| Street address |
300 Feng Lin Road
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platform ID |
GPL13157 |
| Series (1) |
| GSE150971 |
Swarming sample vs non-swarming sample |
|
