|
| Status |
Public on Oct 02, 2009 |
| Title |
Conrtrol Cy3 vs Brain Cy5 (Batch1) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Control
|
| Organism |
Ciona intestinalis |
| Characteristics |
tissue: Cells of larva without brain vesicles
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from cells without brain vesicle was extracted by acid guanidium thiocyanate-phenol-chloroform extraction method.
|
| Label |
Cy3
|
| Label protocol |
Total RNA was labeled with Cy3 using an Agilent Quick-Amp Labeling Kit (Agilent Technologies). The protocols were according to the manufacturer's instructions.
|
| |
|
| Channel 2 |
| Source name |
Brain
|
| Organism |
Ciona intestinalis |
| Characteristics |
tissue: Brain vesicle of larva
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from brain vesicle was extracted by acid guanidium thiocyanate-phenol-chloroform extraction method.
|
| Label |
Cy5
|
| Label protocol |
Total RNA was labeled with Cy5 using an Agilent Quick-Amp Labeling Kit (Agilent Technologies). The protocols were according to the manufacturer's instructions.
|
| |
|
| |
| Hybridization protocol |
Hybridization and washing were performed using GE Hybridization Kit and GE Wash Pack (Agilent Technologies). The protocols were according to the manufacturer's instructions.
|
| Scan protocol |
Hybridized microarrays were scanned on an Agilent Technologies G2565BA Microarray Scanner System.
|
| Description |
RNA quality control with Agilent Bioanalyzer 2100
|
| Data processing |
The intensity of probes was extracted from scanned microarray images using Feature Extraction 10.5 software (Agilent Technologies). All algorithms and parameters used in this analysis were the default condition of the software. Some probes which were judged beyond analysis by Feature Extraction 10.5 software were eliminated from the following analysis.
|
| |
|
| Submission date |
Oct 01, 2009 |
| Last update date |
Oct 01, 2009 |
| Contact name |
Mayuko Hamada |
| E-mail(s) |
hamadam@okayama-u.ac.jp
|
| Organization name |
Okayama University
|
| Lab |
Ushimado Marine Institute
|
| Street address |
130-17, Kashino, Ushimado
|
| City |
Setouchi |
| State/province |
Okayama |
| ZIP/Postal code |
701-4303 |
| Country |
Japan |
| |
|
| Platform ID |
GPL5576 |
| Series (1) |
| GSE18364 |
Transcriptome Analysis of Ciona intestinalis to Identify Genes Preferentially Expressed in the Larval Brain |
|
