|
Status |
Public on Sep 25, 2020 |
Title |
SPL002 |
Sample type |
SRA |
|
|
Source name |
shrek preleukemic thymocytes
|
Organism |
Danio rerio |
Characteristics |
cell type: thymocytes genotype: shrek;Tg(lck:GFP) tumor stage: preleukemic lab of sample collection and sequencing: Frazer lab
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Truseq stranded mRNA library prep (illumina, #20020594)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 3000 |
|
|
Description |
RNA isolated from sorted GFP positive thymocytes from preleukemic Tg(lck:GFP);shrek mutant fish. rawCountMatrix_Frazier.txt.gz Fsample_48
|
Data processing |
First read of Fastq files were aligned to GRCz10 using STARv2.4.2a with esembl GRCz10.91.gtf as guide Counts to GRCz10.91.gtf were generated on the fly by STAR Genome_build: GRCz10 Supplementary_files_format_and_content: txt file with raw counts
|
|
|
Submission date |
Jun 04, 2020 |
Last update date |
Sep 25, 2020 |
Contact name |
Siebe Loontiens |
E-mail(s) |
siebe.loontiens@ugent.be
|
Organization name |
Ghent University
|
Department |
Center for Medical Genetics
|
Street address |
Corneel Heymanslaan 10
|
City |
Gent |
ZIP/Postal code |
9000 |
Country |
Belgium |
|
|
Platform ID |
GPL23274 |
Series (1) |
GSE151816 |
A novel TLX1 driven T-ALL zebrafish model: comparative genomic analysis with other leukemia models |
|
Relations |
BioSample |
SAMN15102187 |
SRA |
SRX8472330 |