|
| Status |
Public on Sep 10, 2010 |
| Title |
F8310P3_REP2 |
| Sample type |
RNA |
| |
|
| Source name |
F8310P3_REP2
|
| Organism |
Medicago truncatula |
| Characteristics |
genotype: F83005.5
development stage: seedlings at 100°Cd after germination (degree day)
|
| Treatment protocol |
LowTemp_P3 - 10° time 670h. Seed are sown and grown at low temperature: 10°C in the dark until the harvesting at 100°Cd after germination
|
| Growth protocol |
hypocotyl - - Sand (SIFRACO quality NE34:SiO2 .99.7%, solid density 2.65 g cm23, mean particle size 200 mm) - 97% hygrometry - 10°c - In the Dark
|
| Extracted molecule |
total RNA |
| Extraction protocol |
F8310P3_REP2:25ug.
|
| Label |
Biotin
|
| Label protocol |
labelling Biotin direct, amplification=yes, cRNA 20 ug.
|
| |
|
| Hybridization protocol |
labelled extract quantity: 15ug
|
| Scan protocol |
GCOS, Biotin:pmt voltage 570nm,650V,laser power 1
|
| Description |
medicago truncatula (f252) - dev.stage (boyes et al. plant cell 2001): seedlings at 100°cd after germination (degree day)
|
| Data processing |
The data were normalized with the gcrma algorithm (Irizarry et al., 2003), available in the Bioconductor package (Gentleman and Carey, 2002).
|
| |
|
| Submission date |
Oct 07, 2009 |
| Last update date |
Jul 28, 2010 |
| Contact name |
sandrine balzergue |
| Organization name |
INRA
|
| Lab |
IRHS
|
| Street address |
Rue G. Morel
|
| City |
Beaucouze |
| ZIP/Postal code |
49000 |
| Country |
France |
| |
|
| Platform ID |
GPL4652 |
| Series (1) |
| GSE18449 |
Identification of molecular factors explaining decreased hypocotyl growth at low temperature |
|
