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Status |
Public on Sep 10, 2010 |
Title |
A1710P3_REP1 |
Sample type |
RNA |
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Source name |
A1710P3_REP1
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Organism |
Medicago truncatula |
Characteristics |
genotype: Jemalong A17 development stage: seedlings at 100°Cd after germination (degree day)
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Treatment protocol |
LowTemp_P3 - 10° time 670h. Seed are sown and grown at low temperature: 10°C in the dark until the harvesting at 100°Cd after germination
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Growth protocol |
hypocotyl - - Sand (SIFRACO quality NE34:SiO2 .99.7%, solid density 2.65 g cm23, mean particle size 200 mm) - 97% hygrometry - 10°C - In the Dark
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Extracted molecule |
total RNA |
Extraction protocol |
A1710P3_REP1:37.6ug.
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Label |
Biotin
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Label protocol |
labelling Biotin direct, amplification=yes, cRNA 20 ug.
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Hybridization protocol |
labelled extract quantity: 15ug
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Scan protocol |
GCOS, Biotin:pmt voltage 570nm,650V,laser power 1
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Description |
medicago truncatula (jemalong) - dev.stage (boyes et al. plant cell 2001): seedlings at 100°cd after germination (degree day), 4049
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Data processing |
The data were normalized with the gcrma algorithm (Irizarry et al., 2003), available in the Bioconductor package (Gentleman and Carey, 2002).
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Submission date |
Oct 07, 2009 |
Last update date |
Jul 28, 2010 |
Contact name |
sandrine balzergue |
Organization name |
INRA
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Lab |
IRHS
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Street address |
Rue G. Morel
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City |
Beaucouze |
ZIP/Postal code |
49000 |
Country |
France |
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Platform ID |
GPL4652 |
Series (1) |
GSE18449 |
Identification of molecular factors explaining decreased hypocotyl growth at low temperature |
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Data table header descriptions |
ID_REF |
ID number |
VALUE |
Normalized data by GCRMA |
Data table |
ID_REF |
VALUE |
AFFX-BioB-3_at |
7.584871524 |
AFFX-BioB-5_at |
7.743249836 |
AFFX-BioB-M_at |
8.53005846 |
AFFX-BioC-3_at |
9.574832425 |
AFFX-BioC-5_at |
9.219097207 |
AFFX-BioDn-3_at |
11.88369739 |
AFFX-BioDn-5_at |
11.36033337 |
AFFX-CreX-3_at |
14.25636401 |
AFFX-CreX-5_at |
14.12247741 |
AFFX-DapX-3_at |
10.86130758 |
AFFX-DapX-5_at |
9.912039289 |
AFFX-DapX-M_at |
10.57738019 |
AFFX-LysX-3_at |
7.004511886 |
AFFX-LysX-5_at |
5.473060081 |
AFFX-LysX-M_at |
5.908890821 |
AFFX-Msa-actin-3_at |
7.441469588 |
AFFX-Msa-actin-5_at |
2.888757169 |
AFFX-Msa-actin-M_at |
7.725350992 |
AFFX-Msa-gapc-3_at |
10.99674783 |
AFFX-Msa-gapc-5_at |
11.49188627 |
Total number of rows: 61278
Table truncated, full table size 1780 Kbytes.
Supplementary file |
Size |
Download |
File type/resource |
GSM459597.CEL.gz |
4.1 Mb |
(ftp)(http) |
CEL |
Processed data included within Sample table |
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