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Sample GSM459597 Query DataSets for GSM459597
Status Public on Sep 10, 2010
Title A1710P3_REP1
Sample type RNA
 
Source name A1710P3_REP1
Organism Medicago truncatula
Characteristics genotype: Jemalong A17
development stage: seedlings at 100°Cd after germination (degree day)
Treatment protocol LowTemp_P3 - 10° time 670h. Seed are sown and grown at low temperature: 10°C in the dark until the harvesting at 100°Cd after germination
Growth protocol hypocotyl - - Sand (SIFRACO quality NE34:SiO2 .99.7%, solid density 2.65 g cm23, mean particle size 200 mm) - 97% hygrometry - 10°C - In the Dark
Extracted molecule total RNA
Extraction protocol A1710P3_REP1:37.6ug.
Label Biotin
Label protocol labelling Biotin direct, amplification=yes, cRNA 20 ug.
 
Hybridization protocol labelled extract quantity: 15ug
Scan protocol GCOS, Biotin:pmt voltage 570nm,650V,laser power 1
Description medicago truncatula (jemalong) - dev.stage (boyes et al. plant cell 2001): seedlings at 100°cd after germination (degree day), 4049
Data processing The data were normalized with the gcrma algorithm (Irizarry et al., 2003), available in the Bioconductor package (Gentleman and Carey, 2002).
 
Submission date Oct 07, 2009
Last update date Jul 28, 2010
Contact name sandrine balzergue
Organization name INRA
Lab IRHS
Street address Rue G. Morel
City Beaucouze
ZIP/Postal code 49000
Country France
 
Platform ID GPL4652
Series (1)
GSE18449 Identification of molecular factors explaining decreased hypocotyl growth at low temperature

Data table header descriptions
ID_REF ID number
VALUE Normalized data by GCRMA

Data table
ID_REF VALUE
AFFX-BioB-3_at 7.584871524
AFFX-BioB-5_at 7.743249836
AFFX-BioB-M_at 8.53005846
AFFX-BioC-3_at 9.574832425
AFFX-BioC-5_at 9.219097207
AFFX-BioDn-3_at 11.88369739
AFFX-BioDn-5_at 11.36033337
AFFX-CreX-3_at 14.25636401
AFFX-CreX-5_at 14.12247741
AFFX-DapX-3_at 10.86130758
AFFX-DapX-5_at 9.912039289
AFFX-DapX-M_at 10.57738019
AFFX-LysX-3_at 7.004511886
AFFX-LysX-5_at 5.473060081
AFFX-LysX-M_at 5.908890821
AFFX-Msa-actin-3_at 7.441469588
AFFX-Msa-actin-5_at 2.888757169
AFFX-Msa-actin-M_at 7.725350992
AFFX-Msa-gapc-3_at 10.99674783
AFFX-Msa-gapc-5_at 11.49188627

Total number of rows: 61278

Table truncated, full table size 1780 Kbytes.




Supplementary file Size Download File type/resource
GSM459597.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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