|
| Status |
Public on Jun 12, 2020 |
| Title |
amniotic fluid P5575 |
| Sample type |
genomic |
| |
|
| Source name |
amniotic fluid fetal cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: amniotic fluid fetal cell
diagnosis: 46,XX,del(X)(p22.31p22.31)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA of fetal cells in amniotic fluid was extracted with using the QIAamp DNA extraction kit (Qiagen, Dusseldorf, Germany)
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix).
|
| |
|
| Hybridization protocol |
DNA was processed as follows. Restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer`s instructions.
|
| Scan protocol |
The arrays were then washed using Affymetrix Fluidics Stations, and scanned using the GeneChip Scanner 3000.
|
| Description |
Hybridized to CytoScan 750K
|
| Data processing |
The array image was acquired using Affymetrix GeneChip® Command Console (AGCC version 4.0.0.1567). Data processing and analysis (CEL file generation, smoothing and segmentation) were used Chromosome Analysis Suite (version 3.3).
Copy number variation status (CNV)
|
| |
|
| Submission date |
Jun 11, 2020 |
| Last update date |
Jun 12, 2020 |
| Contact name |
Hailong Huang |
| Organization name |
Fujian Maternity and Child Health Hospital
|
| Street address |
No.18 Daoshan Road, Gulou District
|
| City |
Fuzhou |
| State/province |
Fujian |
| ZIP/Postal code |
350001 |
| Country |
China |
| |
|
| Platform ID |
GPL18637 |
| Series (1) |
| GSE152288 |
Affymetrix CytoScan 750K data for validation of the BoBs/CNV-seq comparison study |
|
