|
| Status |
Public on Jun 30, 2021 |
| Title |
wild-type mouse fed with high fat diet, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
hippocampus from wild-type mouse fed with high fat diet for 12 months
|
| Organism |
Mus musculus |
| Characteristics |
diabetes status: diabetic
diet: high fat diet
genotype: wild-type
tissue: hippocampus
Sex: male
age: 18 months
|
| Treatment protocol |
Each mouse was anesthetized, transcardially perfused with saline, and hippocamppus was isolated quickly, preserved at –80°C until RNA preparation.
|
| Growth protocol |
Each mouse was maintained in SPF condition and sacrificed at 18 months of age.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Isogen (Nippon Gene) according to the manufacturers’ instructions.
|
| Label |
biotin
|
| Label protocol |
GeneChip® WT PLUS Reagent Kit (Affymetrix) was used to generate amplified and biotinylated sense-strand DNA targets from expressed transcripts (100 ng of total RNA).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 µg of cDNA were hybridized for 17 hr at 45℃ on Affymetrix Mouse Gene 2.0 ST Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000 7G.
|
| Description |
18 month-old male mouse
|
| Data processing |
CEL files were imported into the Transcriptome Analysis Console (TAC) Software 4.0 and CHP files were generated.
|
| |
|
| Submission date |
Jun 15, 2020 |
| Last update date |
Jun 30, 2021 |
| Contact name |
Yusaku Nakabeppu |
| E-mail(s) |
yusaku@bioreg.kyushu-u.ac.jp
|
| Phone |
81-92-642-6800
|
| Organization name |
Medical Institute of Bioregulation, Kyushu University
|
| Department |
Department of Immunobiology and Neuroscience
|
| Lab |
Division of Neurofunctional Genomics
|
| Street address |
3-1-1 Maidashi Higashi-ku
|
| City |
Fukuoka |
| State/province |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE152539 |
A high-fat diet exacerbates the Alzheimer's disease pathology in the hippocampus of the App[NL-F/NL-F] knock-in mouse model |
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