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Sample GSM4617803 Query DataSets for GSM4617803
Status Public on Jun 30, 2021
Title App[NL-F/NL-F] mouse fed with high fat diet, biological rep2
Sample type RNA
 
Source name hippocampus from App[NL-F/NL-F] mouse fed with high fat diet for 12 months
Organism Mus musculus
Characteristics diabetes status: diabetic
diet: high fat diet
genotype: App knock-in
tissue: hippocampus
Sex: male
age: 18 months
Treatment protocol Each mouse was anesthetized, transcardially perfused with saline, and hippocamppus was isolated quickly, preserved at –80°C until RNA preparation.
Growth protocol Each mouse was maintained in SPF condition and sacrificed at 18 months of age.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Isogen (Nippon Gene) according to the manufacturers’ instructions.
Label biotin
Label protocol GeneChip® WT PLUS Reagent Kit (Affymetrix) was used to generate amplified and biotinylated sense-strand DNA targets from expressed transcripts (100 ng of total RNA).
 
Hybridization protocol Following fragmentation, 5.5 µg of cDNA were hybridized for 17 hr at 45℃ on Affymetrix Mouse Gene 2.0 ST Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000 7G.
Description 18 month-old male mouse
Data processing CEL files were imported into the Transcriptome Analysis Console (TAC) Software 4.0 and CHP files were generated.
 
Submission date Jun 15, 2020
Last update date Jun 30, 2021
Contact name Yusaku Nakabeppu
E-mail(s) yusaku@bioreg.kyushu-u.ac.jp
Phone 81-92-642-6800
Organization name Medical Institute of Bioregulation, Kyushu University
Department Department of Immunobiology and Neuroscience
Lab Division of Neurofunctional Genomics
Street address 3-1-1 Maidashi Higashi-ku
City Fukuoka
State/province Fukuoka
ZIP/Postal code 812-8582
Country Japan
 
Platform ID GPL16570
Series (1)
GSE152539 A high-fat diet exacerbates the Alzheimer's disease pathology in the hippocampus of the App[NL-F/NL-F] knock-in mouse model

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
17200001 4.91373
17200003 5.73537
17200005 4.11252
17200007 5.02425
17200009 5.45651
17200011 3.98336
17200013 4.11737
17200015 5.37673
17200017 1.75765
17200019 3.34398
17200021 5.10769
17200023 5.53345
17200025 5.15572
17200027 5.99047
17200029 2.65237
17200031 3.12713
17200033 3.01106
17200035 3.22782
17200037 4.62259
17200039 3.24905

Total number of rows: 41345

Table truncated, full table size 681 Kbytes.




Supplementary file Size Download File type/resource
GSM4617803_App_NL-F_HDF-2.CEL.gz 8.7 Mb (ftp)(http) CEL
GSM4617803_App_NL-F_HDF-2.CHP.gz 254.9 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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