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Status |
Public on Jun 30, 2021 |
Title |
App[NL-F/NL-F] mouse fed with high fat diet, biological rep2 |
Sample type |
RNA |
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Source name |
hippocampus from App[NL-F/NL-F] mouse fed with high fat diet for 12 months
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Organism |
Mus musculus |
Characteristics |
diabetes status: diabetic diet: high fat diet genotype: App knock-in tissue: hippocampus Sex: male age: 18 months
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Treatment protocol |
Each mouse was anesthetized, transcardially perfused with saline, and hippocamppus was isolated quickly, preserved at –80°C until RNA preparation.
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Growth protocol |
Each mouse was maintained in SPF condition and sacrificed at 18 months of age.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using Isogen (Nippon Gene) according to the manufacturers’ instructions.
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Label |
biotin
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Label protocol |
GeneChip® WT PLUS Reagent Kit (Affymetrix) was used to generate amplified and biotinylated sense-strand DNA targets from expressed transcripts (100 ng of total RNA).
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Hybridization protocol |
Following fragmentation, 5.5 µg of cDNA were hybridized for 17 hr at 45℃ on Affymetrix Mouse Gene 2.0 ST Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000 7G.
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Description |
18 month-old male mouse
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Data processing |
CEL files were imported into the Transcriptome Analysis Console (TAC) Software 4.0 and CHP files were generated.
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Submission date |
Jun 15, 2020 |
Last update date |
Jun 30, 2021 |
Contact name |
Yusaku Nakabeppu |
E-mail(s) |
yusaku@bioreg.kyushu-u.ac.jp
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Phone |
81-92-642-6800
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Organization name |
Medical Institute of Bioregulation, Kyushu University
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Department |
Department of Immunobiology and Neuroscience
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Lab |
Division of Neurofunctional Genomics
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Street address |
3-1-1 Maidashi Higashi-ku
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City |
Fukuoka |
State/province |
Fukuoka |
ZIP/Postal code |
812-8582 |
Country |
Japan |
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Platform ID |
GPL16570 |
Series (1) |
GSE152539 |
A high-fat diet exacerbates the Alzheimer's disease pathology in the hippocampus of the App[NL-F/NL-F] knock-in mouse model |
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